Hamburg M D
Science. 1967 May 19;156(3777):973-4. doi: 10.1126/science.156.3777.973.
Intraperitoneal injection of physostigmine in rats produced a retrograde amnesia of a trained task of escaping shock. This amnesic effect was a U-shaped function of the length of the interval between initial training and injection. In all cases, retraining Occurred 30 minutes after injection. A substantial effect was produced by physostigmine if its application was made 30 minutes after training; there was no effect if application and tests were made 1, 2, or 3 days after the original training. When the substance was injected and the rats were retrained 5, 7, or 14 days after the original training, a substantial effect again appeared. These results are similar to those reported in experiments in which another anticholinesterase, diisopropyl fluorophosphate, was applied intracerebrally. The data demonstrate a similar pattern of change of the amnesia with time, and they substantiate the view that neither the place of application nor the brain lesions caused the reported amnesia.
给大鼠腹腔注射毒扁豆碱会导致其对一项逃避电击的训练任务产生逆行性遗忘。这种遗忘效应是初始训练与注射之间间隔时间长度的U形函数。在所有情况下，注射后30分钟进行重新训练。如果在训练后30分钟应用毒扁豆碱，会产生显著效果；如果在初次训练后1天、2天或3天进行应用和测试，则没有效果。当在初次训练后5天、7天或14天注射该物质并对大鼠进行重新训练时，又会出现显著效果。这些结果与在实验中报告的结果相似，在那些实验中，另一种抗胆碱酯酶，即二异丙基氟磷酸，被脑内应用。数据表明遗忘随时间变化的模式相似，并且证实了应用部位和脑损伤均未导致所报告的遗忘这一观点。
