Khan A U
Biochem Biophys Res Commun. 1984 Jul 31;122(2):668-75. doi: 10.1016/s0006-291x(84)80085-6.
Myeloperoxidase in micromolar concentrations reacting with half-millimolar stock solution H2O2 in acetate buffer containing KBr and in 50% D2O (pH + pD = 4.5) at 298 K is shown to generate singlet delta molecular oxygen efficiently. The near infrared electronic emission of singlet oxygen at 1268 nm is detected directly by novel ultrasensitive IR spectrophotometer equipment. The quantum efficiency of singlet oxygen generation by the MPO X Br- X H2O2 reaction is shown to be comparable with that of the standard chemical reaction OCl- X H2O2 at identical peroxide concentrations.
在含有溴化钾的醋酸盐缓冲液中，以及在50%重水（pH + pD = 4.5）的条件下，微摩尔浓度的髓过氧化物酶于298 K与半毫摩尔储备溶液过氧化氢发生反应，结果表明能高效生成单线态δ分子氧。通过新型超灵敏红外分光光度计设备直接检测到了单线态氧在1268 nm处的近红外电子发射。结果表明，在相同的过氧化物浓度下，髓过氧化物酶-溴离子-过氧化氢反应生成单线态氧的量子效率与标准化学反应次氯酸根离子-过氧化氢的量子效率相当。
