Taylor B A, Rowe L
Mol Gen Genet. 1984;195(3):491-9. doi: 10.1007/BF00341452.
Several restriction fragment length variants have been detected among inbred strains using a mouse serum amyloid A cDNA clone. Five variants were shown to segregate as a single genetic unit and were mapped to Chromosome 7 between the glucose phosphate isomerase locus (Gpi-1) and the pink eye dilution locus (p) using recombinant inbred and congenic strains. The finding that no major MspI or BclI restriction fragments were shared between digests of DNAs from a Chromosome 7 congenic strain and its inbred partner, indicate that most, and probably all, sequences detected with the probe are clustered on Chromosome 7. Aneuploid mapping was used to show that the serum amyloid A gene complex (Saa) is proximal to the Chromosome 7 breakpoint in T(7;X)1Ct, a translocation in which the middle third of Chromosome 7 is inserted into the X-chromosome. A survey of inbred strains revealed a single common Saa haplotype and eight rare haplotypes. The complex distribution of 14 different variants suggests that recombination may have played a role in haplotype evolution.
利用小鼠血清淀粉样蛋白A cDNA克隆，在近交系中检测到了几种限制性片段长度变异。其中5种变异显示为作为一个单一遗传单位进行分离，并利用重组近交系和同源近交系，将其定位到7号染色体上磷酸葡萄糖异构酶基因座（Gpi-1）和粉红眼稀释基因座（p）之间。7号染色体同源近交系与其近交亲本的DNA消化产物之间没有共享主要的MspI或BclI限制性片段，这一发现表明，用该探针检测到的大多数（可能是所有）序列都聚集在7号染色体上。利用非整倍体定位表明，血清淀粉样蛋白A基因复合体（Saa）位于T(7;X)1Ct中7号染色体断点的近端，T(7;X)1Ct是一种易位，其中7号染色体的中间三分之一插入到X染色体中。对近交系的调查揭示了一种单一的常见Saa单倍型和8种罕见单倍型。14种不同变异的复杂分布表明，重组可能在单倍型进化中发挥了作用。
