Abe K, Takano H, Ito T
Arch Histol Jpn. 1984 Jun;47(2):121-35. doi: 10.1679/aohc.47.121.
Ligation of the efferent duct in the mouse results in appearance of PAS-positive inclusions in principal cells in the duct of the epididymal body, and the inclusions are considered to be formed by absorption of the luminal PAS-positive material produced in the epididymal head (ABE et al., 1982a). For better understanding of the significance of the inclusions, this study examined morphologic changes in the principal cells after efferent duct cutting, by light and electron microscopy. Electron microscopy revealed that the principal cells containing PAS-positive inclusions are characterized by a nipple-like protrusion--micropapilla--on the luminal surface and by peculiar multivesicular bodies in the supranuclear cytoplasm. The micropapilla, 1-2 micron in height, contains ductules of 0.2-0.5 micron thickness which extends from its tip to the multivesicular bodies. The multivesicular bodies, measuring up to 10 micron in diameter, are larger toward the nucleus. They contain numerous vesicles, 40-100 nm in diameter, and an amorphous material that is similar to the luminal material of the epididymal duct. In addition, larger multivesicular bodies are heterogenous in structure and possess bundles of tubules 40 nm thick. There are dense bodies in the basal cytoplasm under the nucleus which appear to be transformed from the multivesicular bodies. In summary, occurrence of the micropapilla and the giant multivesicular bodies are considered to represent an activated breakdown of the material ingested from the duct lumen.
在小鼠中结扎输出小管会导致附睾体管主细胞中出现PAS阳性包涵体，这些包涵体被认为是由附睾头部产生的管腔PAS阳性物质吸收形成的（ABE等人，1982a）。为了更好地理解这些包涵体的意义，本研究通过光学显微镜和电子显微镜检查了输出小管切断后主细胞的形态变化。电子显微镜显示，含有PAS阳性包涵体的主细胞的特征是在管腔表面有乳头样突起——微乳头，以及在核上细胞质中有特殊的多囊泡体。微乳头高1-2微米，含有从其顶端延伸至多囊泡体的厚度为0.2-0.5微米的细管。多囊泡体直径可达10微米，靠近细胞核处更大。它们含有许多直径为40-100纳米的囊泡以及一种与附睾管腔物质相似的无定形物质。此外，较大的多囊泡体结构不均匀，拥有40纳米厚的管束。在细胞核下方的基底细胞质中有致密体，似乎是由多囊泡体转化而来。总之，微乳头和巨大多囊泡体的出现被认为代表了从管腔摄入物质的激活分解。
