Clare D A, Duong M N, Darr D, Archibald F, Fridovich I
Anal Biochem. 1984 Aug 1;140(2):532-7. doi: 10.1016/0003-2697(84)90204-5.
The usual method of staining polyacrylamide gel electropherograms for superoxide dismutase activity utilizes a photochemical flux of O2- to reduce nitroblue tetrazolium. Superoxide dismutases intercept O2-, preventing formazan production and thus causing achromatic bands. In the presence of H2O2, catalases also yield achromatic bands during this staining procedure. This is due to local elevation of pO2 by the catalatic decomposition of H2O2. O2, in turn, inhibits the reduction of the tetrazolium by O2-. This phenomenon provides a new activity stain for catalase. A previously described activity stain for catalase has also been reexamined and significantly improved.
用于检测超氧化物歧化酶活性的聚丙烯酰胺凝胶电泳图谱的常规染色方法，利用O2-的光化学通量来还原氮蓝四唑。超氧化物歧化酶拦截O2-，阻止甲臜生成，从而产生无色条带。在H2O2存在的情况下，过氧化氢酶在此染色过程中也会产生无色条带。这是由于H2O2的催化分解导致局部pO2升高。反过来，O2会抑制O2-对四唑的还原。这种现象为过氧化氢酶提供了一种新的活性染色方法。之前描述的过氧化氢酶活性染色方法也经过了重新检验并得到了显著改进。
