Buchanan R L, Lewis D F
Appl Environ Microbiol. 1984 Aug;48(2):306-10. doi: 10.1128/aem.48.2.306-310.1984.
Catabolism of carbohydrates has been implicated in the regulation of aflatoxin synthesis. To characterize this effect further, the activities of various enzymes associated with glucose catabolism were determined in Aspergillus parasiticus organisms that were initially cultured in peptone-mineral salts medium and then transferred to glucose-mineral salts and peptone-mineral salts media. After an initial increase in activity, the levels of glucose 6-phosphate dehydrogenase, mannitol dehydrogenase, and malate dehydrogenase were lowered in the presence of glucose. Phosphofructokinase activity was greater in the peptone-grown mycelium, but fructose diphosphatase was largely unaffected by carbon source. Likewise, carbon source had relatively little effect on the activities of pyruvate kinase, malic enzyme, isocitrate-NADP dehydrogenase, and isocitrate-NAD dehydrogenase. The results suggest that glucose may, in part, regulate aflatoxin synthesis via a carbon catabolite repression of NADPH-generating and tricarboxylic acid cycle enzymes.
碳水化合物的分解代谢与黄曲霉毒素合成的调控有关。为了进一步表征这种效应，在最初在蛋白胨 - 矿物盐培养基中培养，然后转移到葡萄糖 - 矿物盐和蛋白胨 - 矿物盐培养基中的寄生曲霉生物体中，测定了与葡萄糖分解代谢相关的各种酶的活性。在活性最初增加之后，在葡萄糖存在下，6 - 磷酸葡萄糖脱氢酶、甘露醇脱氢酶和苹果酸脱氢酶的水平降低。磷酸果糖激酶活性在蛋白胨生长的菌丝体中更高，但果糖二磷酸酶在很大程度上不受碳源的影响。同样，碳源对丙酮酸激酶、苹果酸酶、异柠檬酸 - NADP脱氢酶和异柠檬酸 - NAD脱氢酶的活性影响相对较小。结果表明，葡萄糖可能部分地通过对产生NADPH的酶和三羧酸循环酶的碳分解代谢物阻遏来调节黄曲霉毒素的合成。
