Torres A R, Li M K, Ward D C, Edberg S C
J Clin Microbiol. 1984 Oct;20(4):687-90. doi: 10.1128/jcm.20.4.687-690.1984.
We used the restriction endonuclease HaeIII to differentiate Neisseria gonorrhoeae from other Neisseria species and Branhamella catarrhalis. A total of 16 clinical isolates and four American Type Culture Collection strains of N. gonorrhoeae were resistant to HaeIII digestion, whereas 17 isolates and four American Type Culture Collection strains from eight different bacterial species were susceptible. This resistance was not caused by an enzyme inhibitor. We propose that protection of the HaeIII recognition sequence by methylation is the mechanism of resistance since N. gonorrhoeae DNA became susceptible to digestion when passed in Escherichia coli as part of a plasmid clone.
我们使用限制性内切酶HaeIII来区分淋病奈瑟菌与其他奈瑟菌属物种及卡他布兰汉菌。总共16株临床分离株和4株美国典型培养物保藏中心的淋病奈瑟菌菌株对HaeIII消化具有抗性，而来自8个不同细菌物种的17株分离株和4株美国典型培养物保藏中心菌株则敏感。这种抗性不是由酶抑制剂引起的。我们提出，通过甲基化对HaeIII识别序列的保护是抗性机制，因为淋病奈瑟菌DNA作为质粒克隆的一部分在大肠杆菌中传代时变得易于消化。
