Hawley R G, Shulman M J, Hozumi N
Mol Cell Biol. 1984 Dec;4(12):2565-72. doi: 10.1128/mcb.4.12.2565-2572.1984.
Each of two severely defective mouse kappa-chain genes has acquired a different intracisternal A particle (IAP) element within one of its introns. One IAP element generated 6-base-pair direct repeats upon insertion. In contrast, the other IAP element was not flanked by direct repeats and was missing a single nucleotide from its 3' terminus. Sequence analysis of the latter IAP element demonstrated that its long terminal repeats were not identical. Nevertheless, the long terminal repeats were organized like proviral long terminal repeats, and this IAP element did contain two regions that were analogous to retroviral priming sites for RNA-directed DNA synthesis. The region that corresponded to a retroviral tRNA primer binding site was complementary to the 3' ends of all mammalian phenylalanine tRNAs. These findings are discussed in the context of the presumed mode of transposition of IAP elements involving the reverse transcription of IAP RNA.
两个严重缺陷的小鼠κ链基因中的每一个都在其一个内含子内获得了一个不同的核内A颗粒（IAP）元件。一个IAP元件在插入时产生了6个碱基对的直接重复序列。相比之下，另一个IAP元件两侧没有直接重复序列，并且其3'末端缺失了一个核苷酸。对后一个IAP元件的序列分析表明，其长末端重复序列并不相同。然而，长末端重复序列的组织方式类似于前病毒长末端重复序列，并且这个IAP元件确实包含两个类似于逆转录病毒RNA指导DNA合成的引物位点的区域。与逆转录病毒tRNA引物结合位点相对应的区域与所有哺乳动物苯丙氨酸tRNA的3'末端互补。这些发现是在假定的IAP元件转座模式（涉及IAP RNA的逆转录）的背景下进行讨论的。
