Neville A M, Edwards P A, O'Hare M J
Med Oncol Tumor Pharmacother. 1984;1(2):73-6. doi: 10.1007/BF02934977.
Present methods and systems for the generation of human monoclonal antibodies are briefly reviewed. The specificities of the available reagents are outlined. It would appear that the generation using hybridoma methods of human monoclonal antibodies to human tumor cell surface antigens is a rare event and that methods of in vitro immunostimulation may have to be used if such antibodies are to be reliably produced.
简要回顾了目前用于生成人单克隆抗体的方法和系统。概述了现有试剂的特异性。使用杂交瘤方法生成针对人肿瘤细胞表面抗原的人单克隆抗体似乎是罕见事件，并且如果要可靠地生产此类抗体，可能必须使用体外免疫刺激方法。
