Winters S J, Troen P
J Clin Endocrinol Metab. 1983 Aug;57(2):432-5. doi: 10.1210/jcem-57-2-432.
The pulsatile pattern of LH secretion was studied in six patients with primary testicular failure and six healthy adult men by drawing peripheral blood samples every 10 min for 12 h. Mean basal LH concentrations were increased approximately 6-fold in hypogonadal men. In these patients mean absolute LH pulse amplitude was 3 times that of the controls [106 +/- 27 (SE) ng/ml vs. 31 +/- 2 ng/ml; P less than 0.01] and the frequency of spontaneous LH secretory episodes was increased 2-fold (between-pulse interval of 53 +/- 3 min vs. 107 +/- 8 min; P less than 0.01). Mean pulse amplitude was directly related to mean LH concentration in hypogonadal men (r = 0.94, P less than 0.01), but not in controls (r = 0.38, P = NS). Within each group there was no relationship between circulating testosterone or mean LH levels and LH pulse frequency. This study demonstrates that the increased circulating levels of LH in hypogonadal men are a consequence of an increase in both LH pulse frequency and pulse amplitude. The prolonged, more frequent sampling protocol used revealed a pattern of LH secretion not evident in previous studies. Finally, these data provide further, albeit indirect, evidence that androgens may regulate the intermittent release of LHRH in man.
通过每10分钟采集一次外周血样本，持续12小时，对6例原发性睾丸功能衰竭患者和6名健康成年男性的促黄体生成素（LH）分泌的脉冲模式进行了研究。性腺功能减退男性的平均基础LH浓度增加了约6倍。在这些患者中，平均绝对LH脉冲幅度是对照组的3倍[106±27（标准误）ng/ml对31±2 ng/ml；P<0.01]，自发性LH分泌发作的频率增加了2倍（脉冲间期为53±3分钟对107±8分钟；P<0.01）。性腺功能减退男性的平均脉冲幅度与平均LH浓度直接相关（r = 0.94，P<0.01），但在对照组中无相关性（r = 0.38，P = 无显著性差异）。在每组中，循环睾酮或平均LH水平与LH脉冲频率之间均无关系。本研究表明，性腺功能减退男性中LH循环水平的升高是LH脉冲频率和脉冲幅度增加的结果。所采用的延长、更频繁的采样方案揭示了一种在以往研究中不明显的LH分泌模式。最后，这些数据提供了进一步的、尽管是间接的证据，表明雄激素可能调节人类促性腺激素释放激素（LHRH）的间歇性释放。
