Altmannsberger M, Osborn M, Schäfer H, Schauer A, Weber K
Virchows Arch B Cell Pathol Incl Mol Pathol. 1984;45(2):113-24. doi: 10.1007/BF02889858.
Ten nephroblastomas were investigated by antibodies to intermediate filaments. In seven cases, which in light microscopy were characterized by the presence of blastema and tubules, immunofluorescence microscopy with IF-specific antibodies reveals expression of cytokeratin and vimentin in blastema cells, while tubules were only labelled by the cytokeratin antibodies. This result was independent of whether the conventional cytokeratin antibody or monoclonal antibodies specific for cytokeratin 18 were used. Stroma cells were vimentin-positive. In two cases nephroblastomas were undifferentiated and also lacked tubuli formation. In both these tumors blastema cells were vimentin-positive and cytokeratin-negative. Finally one case of clear cell sarcoma of the kidney could only be labelled by the vimentin antibody. Thus antibodies to intermediate filaments seem to be useful tools to distinguish nephroblastomas from neuroblastomas or rhabdomyosarcomas, especially in cases of metastasis.
运用针对中间丝的抗体对10例肾母细胞瘤进行了研究。在7例光镜下以胚基和小管存在为特征的病例中，使用中间丝特异性抗体进行免疫荧光显微镜检查发现，胚基细胞中细胞角蛋白和波形蛋白均有表达，而小管仅被细胞角蛋白抗体标记。无论使用传统的细胞角蛋白抗体还是针对细胞角蛋白18的单克隆抗体，结果均无差异。基质细胞波形蛋白呈阳性。2例肾母细胞瘤未分化且也缺乏小管形成。在这两种肿瘤中，胚基细胞波形蛋白呈阳性而细胞角蛋白呈阴性。最后，1例肾透明细胞肉瘤仅能被波形蛋白抗体标记。因此，针对中间丝的抗体似乎是区分肾母细胞瘤与神经母细胞瘤或横纹肌肉瘤的有用工具，尤其是在转移病例中。
