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酿酒酵母谷氨酰胺-tRNA合成酶突变的鉴定。

Identification of a glutaminyl-tRNA synthetase mutation Saccharomyces cerevisiae.

作者信息

Mitchell A P, Ludmerer S W

出版信息

J Bacteriol. 1984 May;158(2):530-4. doi: 10.1128/jb.158.2.530-534.1984.

DOI:10.1128/jb.158.2.530-534.1984
PMID:6144664
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215461/
Abstract

Saccharomyces cerevisiae glutaminyl-tRNA synthetase mutants were isolated through systematic screening of tight Gln- derivatives of a leaky glutamine auxotroph. These mutations define a single nuclear gene, GLN4. The gln4-1 mutation is specific for Gln-tRNA synthetase and shows a dosage effect in heterozygous diploids. The wild-type Gln-tRNA synthetase exhibits a Km for glutamine of 25 microM; the gln4-1 mutation increases this value 20-fold. These observations strongly suggest that GLN4 encodes the Gln-tRNA synthetase.

摘要

通过对渗漏型谷氨酰胺营养缺陷型的紧密 Gln-衍生物进行系统筛选，分离出了酿酒酵母谷氨酰胺-tRNA 合成酶突变体。这些突变定义了一个单一的核基因 GLN4。gln4-1 突变对谷氨酰胺-tRNA 合成酶具有特异性，并且在杂合二倍体中表现出剂量效应。野生型谷氨酰胺-tRNA 合成酶对谷氨酰胺的 Km 值为 25μM；gln4-1 突变使该值增加了 20 倍。这些观察结果强烈表明 GLN4 编码谷氨酰胺-tRNA 合成酶。

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Identification of a glutaminyl-tRNA synthetase mutation Saccharomyces cerevisiae.酿酒酵母谷氨酰胺-tRNA合成酶突变的鉴定。
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2
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引用本文的文献

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Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8619-24. doi: 10.1073/pnas.95.15.8619.
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Regulation of glutamine-repressible gene products by the GLN3 function in Saccharomyces cerevisiae.酿酒酵母中GLN3功能对谷氨酰胺可阻遏基因产物的调控
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Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae.酿酒酵母细胞质丝氨酰 - tRNA合成酶编码基因的克隆与鉴定
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本文引用的文献

1
Purification and properties of glutamine synthetase from Saccharomyces cerevisiae.酿酒酵母谷氨酰胺合成酶的纯化及性质
J Biol Chem. 1983 Jan 10;258(1):119-24.
2
Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis.表现出蛋白质合成快速抑制的酵母温度敏感突变体。
J Bacteriol. 1968 Nov;96(5):1664-71. doi: 10.1128/jb.96.5.1664-1671.1968.
3
A mutant of yeast with a defective methionyl-tRNA synthetase.一种甲硫氨酰 - tRNA合成酶有缺陷的酵母突变体。
Genetics. 1969 Mar;61(3):557-66. doi: 10.1093/genetics/61.3.557.
4
Mutants of yeast with temperature-sensitive isoleucyl-tRNA synthetases.具有温度敏感性异亮氨酰 - tRNA合成酶的酵母突变体。
Proc Natl Acad Sci U S A. 1968 Feb;59(2):422-8. doi: 10.1073/pnas.59.2.422.
5
Absence of involvement of glutamine synthetase and of NAD-linked glutamate dehydrogenase in the nitrogen catabolite repression of arginase and other enzymes in Saccharomyces cerevisiae.谷氨酰胺合成酶和NAD连接的谷氨酸脱氢酶不参与酿酒酵母中精氨酸酶和其他酶的氮分解代谢阻遏。
Biochem Biophys Res Commun. 1974 Sep 9;60(1):150-7. doi: 10.1016/0006-291x(74)90185-5.
6
Function and regulation of aminoacyl-tRNA synthetases in prokaryotic and eukaryotic cells.原核细胞和真核细胞中氨酰-tRNA合成酶的功能与调控
Annu Rev Microbiol. 1975;29:215-50. doi: 10.1146/annurev.mi.29.100175.001243.
7
Genetics of borrelidin resistant mutants of Saccharomyces cerivisiae and properties of their threonyl-tRNA-synthetase.酿酒酵母对硼缬氨酸抗性突变体的遗传学及其苏氨酰-tRNA合成酶的特性
Mol Gen Genet. 1976 Aug 10;147(1):39-43. doi: 10.1007/BF00337933.
8
Aminoacyl-tRNA synthetases: general features and recognition of transfer RNAs.氨酰-tRNA合成酶：一般特征及对转运RNA的识别
Annu Rev Biochem. 1979;48:601-48. doi: 10.1146/annurev.bi.48.070179.003125.
9
Synthesis and activation of asparagine in asparagine auxotrophs of Saccharomyces cerevisiae.酿酒酵母天冬酰胺营养缺陷型中天冬酰胺的合成与激活
Eur J Biochem. 1979 Mar;94(2):409-17. doi: 10.1111/j.1432-1033.1979.tb12908.x.