酿酒酵母谷氨酰胺-tRNA合成酶突变的鉴定。
Identification of a glutaminyl-tRNA synthetase mutation Saccharomyces cerevisiae.
作者信息
Mitchell A P, Ludmerer S W
出版信息
J Bacteriol. 1984 May;158(2):530-4. doi: 10.1128/jb.158.2.530-534.1984.
Abstract
Saccharomyces cerevisiae glutaminyl-tRNA synthetase mutants were isolated through systematic screening of tight Gln- derivatives of a leaky glutamine auxotroph. These mutations define a single nuclear gene, GLN4. The gln4-1 mutation is specific for Gln-tRNA synthetase and shows a dosage effect in heterozygous diploids. The wild-type Gln-tRNA synthetase exhibits a Km for glutamine of 25 microM; the gln4-1 mutation increases this value 20-fold. These observations strongly suggest that GLN4 encodes the Gln-tRNA synthetase.
摘要
通过对渗漏型谷氨酰胺营养缺陷型的紧密 Gln-衍生物进行系统筛选，分离出了酿酒酵母谷氨酰胺-tRNA 合成酶突变体。这些突变定义了一个单一的核基因 GLN4。gln4-1 突变对谷氨酰胺-tRNA 合成酶具有特异性，并且在杂合二倍体中表现出剂量效应。野生型谷氨酰胺-tRNA 合成酶对谷氨酰胺的 Km 值为 25μM；gln4-1 突变使该值增加了 20 倍。这些观察结果强烈表明 GLN4 编码谷氨酰胺-tRNA 合成酶。
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1
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J Biol Chem. 1983 Jan 10;258(1):119-24.
2
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J Bacteriol. 1968 Nov;96(5):1664-71. doi: 10.1128/jb.96.5.1664-1671.1968.
3
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Genetics. 1969 Mar;61(3):557-66. doi: 10.1093/genetics/61.3.557.
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Mutants of yeast with temperature-sensitive isoleucyl-tRNA synthetases.具有温度敏感性异亮氨酰 - tRNA合成酶的酵母突变体。
Proc Natl Acad Sci U S A. 1968 Feb;59(2):422-8. doi: 10.1073/pnas.59.2.422.
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