大鼠松果体中P2嘌呤受体的存在。

Presence of P2-purinoceptors in the rat pineal gland.

Ferreira Z S, Cipolla-Neto J, Markus R P

Department of Pharmacology, Universidade de São Paulo, Brazil.

Br J Pharmacol. 1994 May;112(1):107-10. doi: 10.1111/j.1476-5381.1994.tb13037.x.

Abstract

The effects of noradrenaline, ATP, adenylyl-imidodiphosphate (AMP-PNP), adenosine, alpha,beta-methylene-ATP and the P2-purinoceptor antagonist, suramin on N'-acetyl-5-hydroxytryptamine production were studied in cultured denervated rat pineal glands. 2. Noradrenaline (3 nM-1 microM) increased N'-acetyl-5-hydroxytryptamine production as measured both in the gland and the culture medium. 3. In noradrenaline (10 nM)-stimulated pineal glands, ATP (0.03 nM-1 mM) or AMP-PNP (0.1 microM-1 mM) increased N'-acetyl-5-hydroxytryptamine production in a concentration-dependent manner. 4. Alpha,beta-Methylene-ATP at the concentration of 0.1 mM, but not 3 microM, attenuated the enhancement by ATP (0.1 mM) of noradrenaline (10 nM)-induced N'-acetyl-5-hydroxytryptamine production. 5. Suramin (0.1 mM) blocked the potentiating effect of ATP (0.1 mM), but not the potentiating effect of adenosine (0.1 mM) in glands incubated with noradrenaline (10 nM). 6. These findings suggest that the rat pineal gland possesses P2-purinoceptors which when stimulated potentiate the effect of noradrenaline but do not, by themselves, induce an increase in N'-acetyl-5-hydroxytryptamine production.

摘要

研究了去甲肾上腺素、ATP、腺苷酰亚胺二磷酸（AMP-PNP）、腺苷、α,β-亚甲基-ATP以及P2嘌呤受体拮抗剂苏拉明对培养的去神经大鼠松果体中N'-乙酰-5-羟色胺生成的影响。2. 去甲肾上腺素（3 nM - 1 μM）可增加腺体和培养基中所测得的N'-乙酰-5-羟色胺生成量。3. 在去甲肾上腺素（10 nM）刺激的松果体中，ATP（0.03 nM - 1 mM）或AMP-PNP（0.1 μM - 1 mM）以浓度依赖的方式增加N'-乙酰-5-羟色胺生成。4. 0.1 mM浓度的α,β-亚甲基-ATP可减弱ATP（0.1 mM）对去甲肾上腺素（10 nM）诱导的N'-乙酰-5-羟色胺生成的增强作用，但3 μM时无此作用。5. 苏拉明（0.1 mM）可阻断ATP（0.1 mM）的增强作用，但不影响腺苷（0.1 mM）对与去甲肾上腺素（10 nM）一起孵育的腺体的增强作用。6. 这些发现表明，大鼠松果体具有P2嘌呤受体，该受体被刺激时可增强去甲肾上腺素的作用，但自身并不诱导N'-乙酰-5-羟色胺生成增加。