Gorin M B, Tilghman S M
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1351-5. doi: 10.1073/pnas.77.3.1351.
The mouse alpha-fetoprotein mRNA is the product of a single-copy gene whose mRNA coding sequences are represented discontinuously in the genome. Several EcoRI genomic fragments which contain portions of the alpha-fetoprotein gene have been cloned using the EK2 vector lambda gt WES . lambda B. In addition, a mouse genomic library has been screened to obtain a 15.75-kilobase segment of DNA that includes more than 85% of the alpha-fetoprotein coding sequence. Analyses by restriction endonuclease mapping and electron microscopy showed that the mRNA sequence is interrupted by at least 11 intervening sequences which occupy 90% of the cloned DNA.
小鼠甲胎蛋白信使核糖核酸是一个单拷贝基因的产物,其信使核糖核酸编码序列在基因组中呈间断分布。使用EK2载体λgtWES.λB克隆了几个含有甲胎蛋白基因部分片段的EcoRI基因组片段。此外,已对小鼠基因组文库进行筛选,以获得一段15.75千碱基的DNA片段,该片段包含超过85%的甲胎蛋白编码序列。通过限制性内切酶图谱分析和电子显微镜观察表明,信使核糖核酸序列被至少11个间隔序列打断,这些间隔序列占据了克隆DNA的90%。