Bauer K D, Dethlefsen L A
J Histochem Cytochem. 1980 Jun;28(6):493-8. doi: 10.1177/28.6.6156196.
Total RNA content in Chinese hamster ovary and HeLa-S3 cells determined by ultraviolet spectroscopy is compared with the red fluorescence distribution of acridine orange-stained cells observed by flow cytometry. A correlation coefficient of 0.93 is obtained when these methods of estimating RNA content are compared after various RNAse treatments. These data suggest that acridine orange staining effectively quantitates total cellular RNA content when analyzed by flow cytometry, although DNA is also shown to contribute a low but significant background of red fluorescence.
通过紫外光谱法测定的中国仓鼠卵巢细胞和HeLa - S3细胞中的总RNA含量，与通过流式细胞术观察的吖啶橙染色细胞的红色荧光分布进行了比较。在各种核糖核酸酶处理后比较这些估计RNA含量的方法时，获得了0.93的相关系数。这些数据表明，当通过流式细胞术分析时，吖啶橙染色有效地定量了细胞总RNA含量，尽管DNA也显示出对红色荧光有低但显著的背景贡献。
