Nasset E S
Digestion. 1977;16(1-2):108-17. doi: 10.1159/000198061.
Succus entericus from jejunal Thiry-Vella fistulae in fasting conscious dogs was analyzed for amino acids before and after incubation for 15 and 30 min at 38 degrees C in vitro. Total amino acid concentration of succus entericus as collected was about twice that of blood plasma. After incubation, it was increased 76% above control at 15 min and 137% at 30 min. Because succus entericus does not exhibit proteinase activity, and no enzyme or substrate was added before incubation, the increase in amino acid concentration was probably due to hydrolysis of peptides by peptidases normally present in the juice. Volume of secretion and output of amino acids were greatly increased by intravenous administration of glucagon, but the amino acid pattern was quite different from blood plasma. The concentrations of aspartic and glutamic acids were 10- and 5-fold greater than in plasma, and tryptophan was usually undetectable.
对禁食清醒犬空肠Thiry-Vella瘘管的肠液在38℃体外孵育15分钟和30分钟前后的氨基酸进行了分析。采集的肠液中总氨基酸浓度约为血浆的两倍。孵育后，15分钟时比对照组增加了76%，30分钟时增加了137%。由于肠液不表现出蛋白酶活性，且孵育前未添加任何酶或底物，氨基酸浓度的增加可能是由于肠液中正常存在的肽酶对肽的水解作用。静脉注射胰高血糖素可使分泌量和氨基酸输出量大幅增加，但氨基酸模式与血浆有很大不同。天冬氨酸和谷氨酸的浓度比血浆中高10倍和5倍，色氨酸通常检测不到。
