McAuslan B R, Hannan G N, Reilly W, Stewart F H
J Cell Physiol. 1980 Aug;104(2):177-86. doi: 10.1002/jcp.1041040207.
A morphologic and growth control variant of bovine aortal endothelial cells has been isolated and shown to synthesise factor VIII antigen (McAuslan and Reilly '79). The variant also possesses the endothelial surface markers angiotensin converting enzyme and alpha 2-microglobulin. The normal cell synthesises fibronectin and deposits it underneath the cells; the variant also synthesises fibronectin. At least three times more fibronectin is distributed over the upper cell surface of variants. This correlates with the three-fold increased binding of the replication inhibitor Con A and suggests a role of fibronectin in endothelial cell growth control. When stimulated to migrate by CuII ions, the variant leaves deposits of fibronectin in its trail; in contrast, migrating normal cells do not, but they do redistribute their surface fibronectin. As revealed by scanning electron microscopy, variant cells are unusual in that they grow over or under cultured normal endothelial cells. It is proposed that during the process of neovascularisation, variant cells have a special function as lead cells that lay down fibronectin on which an endothelium can become established.
已分离出一种牛主动脉内皮细胞的形态学和生长控制变体，并证明其能合成凝血因子VIII抗原（麦考斯兰和赖利，1979年）。该变体还具有内皮表面标志物血管紧张素转换酶和α2 - 微球蛋白。正常细胞合成纤连蛋白并将其沉积在细胞下方；该变体也合成纤连蛋白。变体细胞上表面分布的纤连蛋白至少是正常细胞的三倍。这与复制抑制剂伴刀豆球蛋白A的结合增加三倍相关，表明纤连蛋白在内皮细胞生长控制中起作用。当被铜离子刺激迁移时，变体在其迁移路径上留下纤连蛋白沉积物；相比之下，迁移的正常细胞则不会，但它们会重新分布其表面的纤连蛋白。扫描电子显微镜显示，变体细胞的不同寻常之处在于它们在培养的正常内皮细胞上方或下方生长。有人提出，在新血管形成过程中，变体细胞作为先导细胞具有特殊功能，可铺设纤连蛋白，在内皮细胞可在其上形成。
