来自大肠杆菌的α-1干扰素对某些细胞功能的影响。

Effect of interferon-alpha 1 from E. coli on some cell functions.

作者信息

Masucci M G, Szigeti R, Klein E, Klein G, Gruest J, Montagnier L, Taira H, Hall A, Nagata S, Weissmann C

出版信息

Science. 1980 Sep 19;209(4463):1431-5. doi: 10.1126/science.6158096.

Abstract

Interferon-alpha 1 from Escherichia coli transformed with a hybrid plasmid containing a human leukocyte complementary DNA insert, induces resistance to virus in appropriate target cells. It also shares the following properties with natural leukocyte interferon (IFN). (i) It enhances natural killing activity of human lymphocytes, (ii) it enhances antibody-dependent cell-mediated cytotoxicity, (iii) it suppresses antigen- and mitogen-induced leukocyte migration inhibition, (iv) it inhibits growth of IFN-sensitive Burkitt lymphoma cells. Since these activities are exhibited by a cloned protein species, they are due to IFN itself and not to other human proteins.

摘要

用含有人白细胞互补DNA插入片段的杂交质粒转化的大肠杆菌产生的α1干扰素，可在适当的靶细胞中诱导抗病毒能力。它还具有以下与天然白细胞干扰素（IFN）相同的特性。（i）它增强人淋巴细胞的自然杀伤活性，（ii）它增强抗体依赖性细胞介导的细胞毒性，（iii）它抑制抗原和有丝分裂原诱导的白细胞迁移抑制，（iv）它抑制对IFN敏感的伯基特淋巴瘤细胞的生长。由于这些活性是由一种克隆的蛋白质表现出来的，所以它们是由IFN本身引起的，而不是由其他人类蛋白质引起的。