Detrisac C J, Mayfield R K, Colwell J A, Garvin A J, Sens D A
J Clin Invest. 1983 Jan;71(1):170-3. doi: 10.1172/jci110747.
As an approach to facilitate the understanding of the progression of diabetic renal disease, we assessed the urine of diabetic patients and normal volunteers for the presence of cells that could be cultured in vitro. The results suggest that both normal control subjects and diabetic patients, without clinically detectable microangiopathy, exfoliate few culturable cells into the urine. In contrast, diabetics with documented retinopathy but without nephropathy exfoliate substantially higher numbers of culturable cells (5.2 cells/100 ml urine), whereas diabetics with both retinopathy and advanced nephropathy exfoliate even greater numbers of culturable cells (50.8 cells/100 ml urine). The cells that are exfoliated and culturable can be divided into five distinct cell types based on morphology at the light microscope level. The exfoliated cells proliferate at clonal density after isolation from urine and are epithelial in appearance. These data suggest that the culture of cells from urine might have diagnostic value as an early indicator of diabetic renal disease and provide a convenient, noninvasive new source of human kidney epithelial cells.
作为一种促进对糖尿病肾病进展理解的方法，我们评估了糖尿病患者和正常志愿者尿液中可在体外培养的细胞的存在情况。结果表明，正常对照受试者和无临床可检测微血管病变的糖尿病患者，尿液中脱落的可培养细胞很少。相比之下，有视网膜病变但无肾病的糖尿病患者脱落的可培养细胞数量显著更高（5.2个细胞/100毫升尿液），而既有视网膜病变又有晚期肾病的糖尿病患者脱落的可培养细胞数量甚至更多（50.8个细胞/100毫升尿液）。根据光学显微镜水平的形态学，脱落并可培养的细胞可分为五种不同的细胞类型。从尿液中分离后，脱落的细胞以克隆密度增殖，外观为上皮细胞。这些数据表明，尿液细胞培养作为糖尿病肾病的早期指标可能具有诊断价值，并提供了一种方便、无创的人类肾上皮细胞新来源。
