双链纤连蛋白cDNA的序列重排和重复可能发生在禽成髓细胞瘤病毒逆转录酶和大肠杆菌DNA聚合酶I进行cDNA合成的过程中。
Sequence rearrangement and duplication of double stranded fibronectin cDNA probably occurring during cDNA synthesis by AMV reverse transcriptase and Escherichia coli DNA polymerase I.
作者信息
Fagan J B, Pastan I, de Crombrugghe B
出版信息
Nucleic Acids Res. 1980 Jul 11;8(13):3055-64. doi: 10.1093/nar/8.13.3055.
Abstract
Two cloned cDNAs derived from the mRNA for cell fibronectin have been sequenced, providing evidence that transcription with AMV reverse transcriptase or Escherichia coli DNA polymerase I may not always result in double stranded cDNA that is exactly homologous with its mRNA template. Instead, the sequences of these cloned cDNAs are consistent with the duplication and rearrangement of sequences during synthesis of double stranded cDNA.
摘要
来自细胞纤连蛋白mRNA的两个克隆cDNA已被测序，这表明用禽成髓细胞瘤病毒（AMV）逆转录酶或大肠杆菌DNA聚合酶I进行转录时，不一定总能产生与其mRNA模板完全同源的双链cDNA。相反，这些克隆cDNA的序列与双链cDNA合成过程中序列的重复和重排是一致的。
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