• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

双链纤连蛋白cDNA的序列重排和重复可能发生在禽成髓细胞瘤病毒逆转录酶和大肠杆菌DNA聚合酶I进行cDNA合成的过程中。

Sequence rearrangement and duplication of double stranded fibronectin cDNA probably occurring during cDNA synthesis by AMV reverse transcriptase and Escherichia coli DNA polymerase I.

作者信息

Fagan J B, Pastan I, de Crombrugghe B

出版信息

Nucleic Acids Res. 1980 Jul 11;8(13):3055-64. doi: 10.1093/nar/8.13.3055.

DOI:10.1093/nar/8.13.3055
PMID:6159581
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324144/
Abstract

Two cloned cDNAs derived from the mRNA for cell fibronectin have been sequenced, providing evidence that transcription with AMV reverse transcriptase or Escherichia coli DNA polymerase I may not always result in double stranded cDNA that is exactly homologous with its mRNA template. Instead, the sequences of these cloned cDNAs are consistent with the duplication and rearrangement of sequences during synthesis of double stranded cDNA.

摘要

来自细胞纤连蛋白mRNA的两个克隆cDNA已被测序，这表明用禽成髓细胞瘤病毒（AMV）逆转录酶或大肠杆菌DNA聚合酶I进行转录时，不一定总能产生与其mRNA模板完全同源的双链cDNA。相反，这些克隆cDNA的序列与双链cDNA合成过程中序列的重复和重排是一致的。

相似文献

1
Sequence rearrangement and duplication of double stranded fibronectin cDNA probably occurring during cDNA synthesis by AMV reverse transcriptase and Escherichia coli DNA polymerase I.双链纤连蛋白cDNA的序列重排和重复可能发生在禽成髓细胞瘤病毒逆转录酶和大肠杆菌DNA聚合酶I进行cDNA合成的过程中。
Nucleic Acids Res. 1980 Jul 11;8(13):3055-64. doi: 10.1093/nar/8.13.3055.
2
[Enzymatic synthesis and molecular cloning of the pigeon alpha-globin structural gene].[鸽α-珠蛋白结构基因的酶促合成与分子克隆]
Mol Biol (Mosk). 1981 Nov-Dec;15(6):1224-33.
3
Apparent allosterism by avian myeloblastosis virus reverse transcriptase and E. coli DNA polymerase I.禽成髓细胞瘤病毒逆转录酶和大肠杆菌DNA聚合酶I的明显变构效应
Nucleic Acids Res. 1979 Mar;6(3):1189-201. doi: 10.1093/nar/6.3.1189.
4
Reverse transcriptase-associated RNase H. Part IV. Pyrophosphate does not inhibit RNase H activity of AMV DNA polymerase.逆转录酶相关核糖核酸酶H。第四部分。焦磷酸不抑制禽成髓细胞瘤病毒DNA聚合酶的核糖核酸酶H活性。
Biochem Biophys Res Commun. 1979 Dec 14;91(3):892-9. doi: 10.1016/0006-291x(79)91963-6.
5
The influence of a double-stranded hindrance on DNA synthesis performed by DNA polymerase alpha, T4 DNA polymerase, DNA polymerase I (Klenow fragment) and AMV reverse transcriptase.双链阻碍对由DNA聚合酶α、T4 DNA聚合酶、DNA聚合酶I（克列诺片段）和禽成髓细胞瘤病毒逆转录酶所进行的DNA合成的影响。
FEBS Lett. 1988 Feb 8;228(1):144-8. doi: 10.1016/0014-5793(88)80604-5.
6
Intrinsic properties of reverse transcriptase in reverse transcription. Associated RNase H is essentially regarded as an endonuclease.逆转录过程中逆转录酶的内在特性。相关的核糖核酸酶H本质上被视为一种核酸内切酶。
J Biol Chem. 1989 Nov 5;264(31):18808-17.
7
Gene specific priming of complementary DNA synthesis.基因特异性引发互补DNA合成。
Mol Biol Rep. 1976 Nov;3(2):167-73. doi: 10.1007/BF00423231.
8
RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.禽成髓细胞瘤病毒逆转录酶用于合成反互补DNA时所使用的RNA引物。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1316-20. doi: 10.1073/pnas.77.3.1316.
9
Characterization of the cDNA synthesized by avian retrovirus reverse transcriptase using 35 S avian myeloblastosis virus RNA and an exogenous bovine primer tRNA.使用35S禽成髓细胞瘤病毒RNA和外源牛引物tRNA对禽逆转录病毒逆转录酶合成的cDNA进行表征。
FEBS Lett. 1988 Mar 28;230(1-2):61-6. doi: 10.1016/0014-5793(88)80642-2.
10
Temperature-dependent template switching during in vitro cDNA synthesis by the AMV-reverse transcriptase.禽成髓细胞瘤病毒逆转录酶在体外cDNA合成过程中依赖温度的模板转换
Nucleic Acids Res. 1992 Oct 25;20(20):5443-50. doi: 10.1093/nar/20.20.5443.

引用本文的文献

1
Developmental biochemistry of cottonseed embryogenesis and germination XVIII cDNA and amino acid sequences of members of the storage protein families.棉籽胚胎发生和萌发的发育生物化学 XVIII 贮藏蛋白家族成员的 cDNA 和氨基酸序列。
Plant Mol Biol. 1986 Nov;7(6):475-89. doi: 10.1007/BF00020331.
2
Analysis of transcribed human endogenous retrovirus W env loci clarifies the origin of multiple sclerosis-associated retrovirus env sequences.转录的人类内源性逆转录病毒W env基因座分析阐明了多发性硬化症相关逆转录病毒env序列的起源。
Retrovirology. 2009 Apr 15;6:37. doi: 10.1186/1742-4690-6-37.
3
Expression pattern analysis of transcribed HERV sequences is complicated by ex vivo recombination.转录的人内源性逆转录病毒（HERV）序列的表达模式分析因体外重组而变得复杂。
Retrovirology. 2007 Jun 6;4:39. doi: 10.1186/1742-4690-4-39.
4
A mercury-thiol affinity system for rapid generation of overlapping labeled DNA fragments for DNA sequencing.一种用于快速生成用于DNA测序的重叠标记DNA片段的汞-硫醇亲和系统。
Nucleic Acids Res. 1982 Jul 10;10(13):4009-25. doi: 10.1093/nar/10.13.4009.
5
Nucleotide sequence of cloned cDNAs encoding human preproparathyroid hormone.编码人甲状旁腺激素原前体的克隆cDNA的核苷酸序列。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7365-9. doi: 10.1073/pnas.78.12.7365.
6
18S defective interfering RNA of Semliki Forest virus contains a triplicated linear repeat.塞姆利基森林病毒的18S缺陷干扰RNA包含一个三倍线性重复序列。
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5353-7. doi: 10.1073/pnas.78.9.5353.
7
Primary structure and gene organization of the middle-component RNA of cowpea mosaic virus.豇豆花叶病毒中间组分RNA的一级结构和基因组织
EMBO J. 1983;2(6):941-6. doi: 10.1002/j.1460-2075.1983.tb01525.x.
8
Isolation of genomic DNA clones spanning the entire fibronectin gene.跨越整个纤连蛋白基因的基因组DNA克隆的分离。
Proc Natl Acad Sci U S A. 1983 Jan;80(1):46-50. doi: 10.1073/pnas.80.1.46.
9
Nucleotide sequence encoding the precursor of the small subunit of ribulose 1,5-bisphosphate carboxylase from Lemna gibba L.G-3.编码浮萍（Lemna gibba L.G-3）1,5-二磷酸核酮糖羧化酶小亚基前体的核苷酸序列。
Nucleic Acids Res. 1983 Nov 25;11(22):8051-61. doi: 10.1093/nar/11.22.8051.
10
Construction of infectious potato spindle tuber viroid cDNA clones.感染性马铃薯纺锤块茎类病毒cDNA克隆的构建。
Nucleic Acids Res. 1983 Oct 11;11(19):6821-35. doi: 10.1093/nar/11.19.6821.

本文引用的文献

1
Isolation and characterization of a protein that stimulates DNA synthesis from avian myeloblastosis virus.从禽成髓细胞瘤病毒中分离并鉴定一种刺激DNA合成的蛋白质。
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2331-5. doi: 10.1073/pnas.69.8.2331.
2
Ribonucleic acid directed deoxyribonucleic acid synthesis by the purified deoxyribonucleic acid polymerase of Rous sarcoma virus. Characterization of the enzymatic product.劳斯肉瘤病毒纯化的脱氧核糖核酸聚合酶催化的核糖核酸指导的脱氧核糖核酸合成。酶促产物的特性
Biochemistry. 1972 Jun 6;11(12):2343-51. doi: 10.1021/bi00762a021.
3
A general method for inserting specific DNA sequences into cloning vehicles.一种将特定DNA序列插入克隆载体的通用方法。
Gene. 1976;1(1):81-92. doi: 10.1016/0378-1119(76)90008-1.
4
Chemical synthesis of restriction enzyme recognition sites useful for cloning.用于克隆的限制性内切酶识别位点的化学合成
Science. 1977 Apr 8;196(4286):177-80. doi: 10.1126/science.847463.
5
The use of thin acrylamide gels for DNA sequencing.用于DNA测序的薄丙烯酰胺凝胶的使用。
FEBS Lett. 1978 Mar 1;87(1):107-10. doi: 10.1016/0014-5793(78)80145-8.
6
Partial purification and characterization of the messenger RNA for cell fibronectin.细胞纤连蛋白信使核糖核酸的部分纯化与特性分析
Nucleic Acids Res. 1979 Aug 10;6(11):3471-80. doi: 10.1093/nar/6.11.3471.
7
Microfilament bundles and cell shape are related to adhesiveness to substratum and are dissociable from growth control in cultured fibroblasts.微丝束和细胞形态与对基质的粘附性有关，并且在培养的成纤维细胞中可与生长控制分离。
Cell. 1977 Mar;10(3):375-80. doi: 10.1016/0092-8674(77)90024-1.
8
A detailed model of reverse transcription and tests of crucial aspects.逆转录的详细模型及关键方面的测试
Cell. 1979 Sep;18(1):93-100. doi: 10.1016/0092-8674(79)90357-x.
9
No more than seven interruptions in the ovalbumin gene: comparison of genomic and double-stranded cDNA sequences.卵清蛋白基因中的中断不超过七个：基因组序列与双链cDNA序列的比较。
Nucleic Acids Res. 1979 Sep 25;7(2):321-34. doi: 10.1093/nar/7.2.321.
10
DNA sequence of the mini-insertion IS2--6 and its relation to the sequence of IS2.小插入序列IS2--6的DNA序列及其与IS2序列的关系。
Nature. 1978 Oct 19;275(5681):611-7. doi: 10.1038/275611a0.