人类成纤维细胞中的两种干扰素信使核糖核酸：体外翻译及大肠杆菌克隆研究
Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies.
作者信息
Weissenbach J, Chernajovsky Y, Zeevi M, Shulman L, Soreq H, Nir U, Wallach D, Perricaudet M, Tiollais P, Revel M
出版信息
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7152-6. doi: 10.1073/pnas.77.12.7152.
Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in Escherichia coli plasmid pBR322. The major fibroblast interferon (Hu IFN-beta 1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 Mr protein. The second interferon mRNA species (Hu IFN-beta 2) is 14S, about 1300 nucleotides long, and codes for another protein of 23,000-26,000 Mr. The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI.rC), but IFN-beta 2 mRNA is induced to about 10% in cells by cycloheximide treatment alone whereas under these conditions IFN-beta 1 is not induced.
从人成纤维细胞中分离出两种产生生物活性干扰素的信使核糖核酸（mRNA），并通过大小分级分离和在大肠杆菌质粒pBR322中克隆进行研究。主要的成纤维细胞干扰素（Hu IFN-β1）由两种mRNA中较小的一种编码，即11S的物种，长900个核苷酸，在无细胞系统中产生一种20,000道尔顿的蛋白质。第二种干扰素mRNA物种（Hu IFN-β2）为14S，约1300个核苷酸长，编码另一种分子量为23,000 - 26,000道尔顿的蛋白质。这两种干扰素mRNA不发生交叉杂交。两者都由聚（rI.rC）诱导，但单独用环己酰亚胺处理时，IFN-β2 mRNA在细胞中被诱导至约10%，而在这些条件下IFN-β1不被诱导。
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