Nielsen J T
Genetics. 1982 Nov;102(3):571-82. doi: 10.1093/genetics/102.3.571.
Pancreatic amylase in the mouse displays considerable quantitative genetic variation. Agar gel electrophoresis reveals that homozygous animals have either one form of the enzyme, type A, or two forms, type AB. Only few animals have been found that contradict this statement, namely among Mus musculus castaneous from Thailand, which has a single-banded B type. Double-banded homozygous specimens of various origins have different relative proportions of the two isoenzymes. By measuring the A:B ratios in such animals, a number of distinct haplotypes or amylase complexes, determining ratios ranging from 61% A:39%, B to 12% A:88% B, have been recognized. These complexes differ also with respect to the total amount of amylase produced. If the reference stock C3H/As is given the value 1, then other haplotypes have values ranging from 1.0 to 0.27. Nineteen amylase haplotypes have been established in congenic lines on a C3H/As background. Some of these lines contain at least four active pancreatic amylase structural genes and breeding experiments have demonstrated that the genetic elements regulating total amylase production and relative proportions of the isoenzymes are located within the amylase complex, cis-acting, and very closely linked to the structural genes.
小鼠中的胰腺淀粉酶表现出相当大的数量遗传变异。琼脂凝胶电泳显示，纯合动物要么有一种酶形式，即A 型，要么有两种形式，即AB 型。仅发现少数动物与这一说法相悖，即在来自泰国的栗褐小家鼠中，其具有单带B 型。不同来源的双带纯合标本中两种同工酶的相对比例不同。通过测量此类动物中的A:B 比值，已识别出许多不同的单倍型或淀粉酶复合物，其比值范围从61% A:39% B 到12% A:88% B。这些复合物在产生的淀粉酶总量方面也有所不同。如果将参考品系C3H/As 的值设定为1，那么其他单倍型的值范围为1.0 至0.27。在C3H/As 背景的近交系中已建立了19 种淀粉酶单倍型。其中一些品系包含至少四个活跃的胰腺淀粉酶结构基因，育种实验表明，调节淀粉酶总产量和同工酶相对比例的遗传元件位于淀粉酶复合物内，顺式作用，且与结构基因紧密连锁。
