Ying S Y, Ling N, Böhlen P, Guillemin R
Endocrinology. 1981 Apr;108(4):1206-15. doi: 10.1210/endo-108-4-1206.
A newly discovered small peptide purified from rat follicular fluid stimulates the pituitary to release FSH and LH in vitro as well as in vivo. Dialysates of crude acid extracts of ovarian follicular tissue and fluid from rats pretreated with PMS gonadotropin stimulate the secretion of both LH and FSH, but not PRL, GH, or TSH, in a pituitary monolayer culture system. This stimulating factor, named gonadocrinin for operational facility, is smaller than 3500 daltons; its biological activity disappears after treatment with trypsin. Gonadocrinin is not recognized by two-antisera binding the decapeptide LRF even though D-Phe2,D-Trp6-LR, an LRF analog antagonist, competitively inhibits the activity of ovarian gonadocrinin. Cultured rat granulosa cells also secret substances with gonadocrinin activity in vitro, indicating that the granulosa cells probably are in vivo the source of gonadocrinin. A crude preparation of gonadocrinin given iv to rats on the second day of diestrus induced secretion of LH comparable to that produced by a 250-ng LRF injection. Gonadocrinin has chemical characteristics different from those of LRF. When purified gonadocrinin or LRF was applied to an identical isocratic high pressure liquid chromatography system, LRF was eluted at a position different from that of gonadocrinin, indicating that, chemically, gonadocrinin is not identical to the hypothalamic decapeptide, LRF.
一种新发现的从大鼠卵泡液中纯化出的小肽，在体外和体内均能刺激垂体释放促卵泡激素（FSH）和促黄体生成素（LH）。用孕马血清促性腺激素（PMS）预处理大鼠后，其卵巢卵泡组织和卵泡液的粗酸提取物的透析液，在垂体单层培养系统中能刺激LH和FSH的分泌，但不刺激催乳素（PRL）、生长激素（GH）或促甲状腺激素（TSH）的分泌。为便于操作，这种刺激因子被命名为促性腺素；其分子量小于3500道尔顿；用胰蛋白酶处理后其生物活性消失。尽管促性腺素释放激素（LRF）类似物拮抗剂D - Phe2，D - Trp6 - LRF能竞争性抑制卵巢促性腺素的活性，但两种能结合十肽LRF的抗血清却不能识别促性腺素。培养的大鼠颗粒细胞在体外也分泌具有促性腺素活性的物质，这表明颗粒细胞可能是体内促性腺素的来源。在动情间期第二天给大鼠静脉注射促性腺素的粗制品，所诱导的LH分泌量与注射250 ng LRF所产生的分泌量相当。促性腺素具有与LRF不同的化学特性。当将纯化的促性腺素或LRF应用于相同的等度高压液相色谱系统时，LRF的洗脱位置与促性腺素不同，这表明在化学性质上，促性腺素与下丘脑十肽LRF并不相同。
