通过与抗免疫球蛋白偶联的酶进行固相抗体检测。
Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin.
作者信息
Leinikki P, Pässilä S
出版信息
J Clin Pathol. 1976 Dec;29(12):1116-20. doi: 10.1136/jcp.29.12.1116.
Abstract
A solid phase antibody assay by means of alkaline phosphatase conjugated to antiimmunoglobulin is described. Specially designed microcuvettes were sensitized with influenza A antigen, and antibodies bound to it were assayed by anti-IgG alkaline phosphatase conjugate in a semiautomated photometer equipped with a programmable calculator. The sensitivity was found to be 200 times higher than HI- or CF-techniques, and the interassay variation was so small that twofold changes in antobody activity could be regarded as significant. Results from vaccinees indicated that serum samples could be collected at intervals of three to six days only to reach a serological diagnosis in clinical patients.
摘要
本文描述了一种通过与抗免疫球蛋白偶联的碱性磷酸酶进行的固相抗体检测方法。特制的微量比色皿用甲型流感抗原致敏，与之结合的抗体通过抗IgG碱性磷酸酶偶联物在配备可编程计算器的半自动光度计中进行检测。结果发现，其灵敏度比血凝抑制试验（HI）或补体结合试验（CF）技术高200倍，且批间差异非常小，以至于抗体活性两倍的变化都可视为有显著意义。疫苗接种者的结果表明，临床患者要进行血清学诊断，血清样本只能每隔三到六天采集一次。
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