Barber C
Microbios. 1980;29(116):89-94.
A profusion of proteins with heterologous serological specificities was synthesized by S. typhimurium grown on artificial media; accordingly, sera prepared in rabbits with these proteins displayed an abundance of antibodies reacting, in agar gel, against numerous heterologous proteins. the absorption of the sera with different Enterobacterial proteins proved that the S. typhimurium proteins are a mixture of specific proteins, and common E. coli and Salmonellae determinants; in addition, a group of strongly cross-precipitating proteins common to S. typhimurium and S. choleraesuis and to S. typhimurium and S. kentucky were identified that were not present in the proteins common to S. enteritidis, S. typhi and E. coli, or in the S. paratyphi A proteins used absorptions. The specific proteins of S. typhimurium were synthesized on artificial media in, apparently, smaller amounts than the common proteins; their role in the protection of mice against infection with their natural pathogen was, however, proof of their specificity and contrasted with the ineffectiveness, in protecting the mice, of the common proteins.
在人工培养基上生长的鼠伤寒沙门氏菌合成了大量具有异源血清学特异性的蛋白质；因此，用这些蛋白质在兔体内制备的血清显示出大量抗体，在琼脂凝胶中能与众多异源蛋白质发生反应。用不同肠道细菌蛋白质对血清进行吸收试验证明，鼠伤寒沙门氏菌蛋白质是特异性蛋白质、大肠杆菌和沙门氏菌共同决定簇的混合物；此外，还鉴定出一组鼠伤寒沙门氏菌与猪霍乱沙门氏菌以及鼠伤寒沙门氏菌与肯塔基沙门氏菌共有的强交叉沉淀蛋白，这些蛋白在肠炎沙门氏菌、伤寒沙门氏菌和大肠杆菌的共有蛋白中不存在，在用甲型副伤寒沙门氏菌蛋白进行吸收试验时也不存在。鼠伤寒沙门氏菌的特异性蛋白质在人工培养基上合成的量显然比共有蛋白少；然而，它们在保护小鼠免受其天然病原体感染方面的作用证明了它们的特异性，这与共有蛋白在保护小鼠方面的无效形成了对比。
