Tada N, Kimura S, Binari R, Liu Y, Hämmerling U
Immunogenetics. 1981;13(6):475-81. doi: 10.1007/BF00343715.
Immunizations of C57BL/6 and A mice with IgA derived from the I/St mouse strain yield alloantisera which detect two allotypic determinants of immunoglobulin A. The two determinants display discrete strain distributions. The first, identified by the alloantiserum C57BL/6 anti-IgA of I/St strain hybridoma ID150, follows the Ighc haplotype, and the second, identified by the alloantiserum A anti-IgA of I/St strain hybridoma ID150, correlates with Ighc and Ighb haplotypes. Absorption with monoclonal IgM, which has the same idiotype as the ID150 IgA clone, removed idiotype-specific antibodies from both alloantisera. The remaining antibodies are directed against determinants associated with the alpha chain constant region, as shown by absorption with monoclonal IgA. By use of recombinant inbred strains of mice and mice congenic at the Igh locus, the loci controlling both C alpha allotypic determinants have been mapped to the Igh region on chromosome 12.
用源自I/St小鼠品系的IgA对C57BL/6和A小鼠进行免疫接种，可产生同种抗血清，该血清可检测免疫球蛋白A的两个同种异型决定簇。这两个决定簇呈现出不同的品系分布。第一个决定簇由I/St品系杂交瘤ID150的同种抗血清C57BL/6抗IgA识别，遵循Ighc单倍型；第二个决定簇由I/St品系杂交瘤ID150的同种抗血清A抗IgA识别，与Ighc和Ighb单倍型相关。用与ID150 IgA克隆具有相同独特型的单克隆IgM进行吸收，可从两种同种抗血清中去除独特型特异性抗体。如用单克隆IgA吸收所示，剩余的抗体针对与α链恒定区相关的决定簇。通过使用重组近交系小鼠和在Igh基因座上的同源小鼠，控制两个Cα同种异型决定簇的基因座已被定位到12号染色体上的Igh区域。
