Bramwell V H, Crowther D, Gallagher J, Stoddart R W
Br J Cancer. 1982 Oct;46(4):582-92. doi: 10.1038/bjc.1982.242.
Fluorescein conjugated lectins have been used as histochemical stains in lymph node sections from 22 patients with non-Hodgkin's Lymphoma. Variations in the distribution and structure of glycoprotein sequences between the different types of lymphoma, and also normal nodes, have been detected. The lectin-binding patterns of neoplastic lymphocytes of small cell lymphomas, both follicular and diffuse, suggested a predominance of sialylated glycopeptides, as in normal small lymphocytes of the mantle zone of germinal centres. In contrast, the staining patterns of large cell follicular and diffuse lymphomas showed a greater diversity of carbohydrate structure, with enhanced cytoplasmic staining and increased numbers of incomplete oligosaccharide sequences. Heterogeneity of staining, together with reduced sialic acid expression at all cellular sites was a common feature of lymphoblastic lymphomas and seemed to be linked with a poor prognosis. The extracellular matrix of small cell follicular lymphomas showed altered saccharide content, but retained some degree of organization. The large cell follicular lymphomas were characterized by a prominent disorderly matrix, with staining characteristics which suggested shedding of surface membrane from component cells. The loss or disordered production of the normal extracellular matrix may reflect a breakdown of control mechanisms within neoplastic follicles.
荧光素结合凝集素已被用作22例非霍奇金淋巴瘤患者淋巴结切片的组织化学染色剂。已检测到不同类型淋巴瘤以及正常淋巴结之间糖蛋白序列分布和结构的差异。滤泡性和弥漫性小细胞淋巴瘤的肿瘤淋巴细胞的凝集素结合模式表明，唾液酸化糖肽占优势，就像生发中心套区的正常小淋巴细胞一样。相比之下，大细胞滤泡性和弥漫性淋巴瘤的染色模式显示碳水化合物结构具有更大的多样性，细胞质染色增强，不完全寡糖序列数量增加。染色异质性以及所有细胞部位唾液酸表达降低是淋巴母细胞淋巴瘤的共同特征，似乎与预后不良有关。小细胞滤泡性淋巴瘤的细胞外基质显示糖含量改变，但仍保留一定程度的组织结构。大细胞滤泡性淋巴瘤的特征是基质明显紊乱，染色特征表明成分细胞表面膜脱落。正常细胞外基质的丧失或无序产生可能反映了肿瘤滤泡内控制机制的破坏。
