Züñiga M C, D'Eustachio P, Ruddle N H
Proc Natl Acad Sci U S A. 1982 May;79(9):3015-9. doi: 10.1073/pnas.79.9.3015.
We have examined the arrangement of immunoglobulin heavy chain constant (CH) and joining (JH) region genes in murine T cell hybrid lines and in T lymphomas. CH genes derived from both parental cell types were present in all hybrids for which polymorphism in sequences flanking CH genes permitted us to distinguish parental CH genes. All T lymphomas and T cell hybrids retained the C alpha gene in germ-line configuration and all but one cell line had germ-line C mu genes. Novel DNA fragments reactive with JH probes were observed in six of nine T cell hybrids, as well as in two T lymphomas, WEHI7.1 and YAC-1, but not in the fusion parent, BW5147. No RNA homologous to C gamma 2b, C alpha, or lambda genes was detected in any of the T cell lines. T cell lines contained poly(A)+ RNA homologous to a C mu cDNA probe. More importantly, in several cell lines the C mu RNAs were associated with membrane-bound polyribosomes. These results suggest that both JH rearrangements and C mu RNA production occur in at least some mature, antigen-specific T cells. They may therefore reflect events in normal T cell development and function related to those involved in the generation of the T receptor for antigen.
我们研究了小鼠T细胞杂交系和T淋巴瘤中免疫球蛋白重链恒定区（CH）和连接区（JH）基因的排列。对于CH基因侧翼序列存在多态性从而使我们能够区分亲代CH基因的所有杂交系，均存在源自两种亲代细胞类型的CH基因。所有T淋巴瘤和T细胞杂交系均以种系构型保留Cα基因，除一个细胞系外，所有细胞系均具有种系Cμ基因。在9个T细胞杂交系中的6个以及2个T淋巴瘤（WEHI7.1和YAC-1）中观察到了与JH探针反应的新DNA片段，但在融合亲代BW5147中未观察到。在任何T细胞系中均未检测到与Cγ2b、Cα或λ基因同源的RNA。T细胞系含有与Cμ cDNA探针同源的聚腺苷酸化（poly(A)+）RNA。更重要的是，在几个细胞系中，Cμ RNA与膜结合的多核糖体相关。这些结果表明，JH重排和Cμ RNA产生至少在一些成熟的、抗原特异性T细胞中发生。因此，它们可能反映了正常T细胞发育和功能中与抗原T受体产生相关的事件。
