Pestalozzi D M, Bühler R, von Wartburg J P, Hess M
Gastroenterology. 1983 Nov;85(5):1011-6.
To elucidate the possible role of alcohol dehydrogenase in the generation of damages caused by alcohol abuse, it is important to know the exact localization of this enzyme. Anti-alcohol dehydrogenase antibodies were used to localize the enzyme in human gastrointestinal tissues. Alcohol dehydrogenase was detectable in the mucosa of all parts of the gastrointestinal tract. There is a notable decrease in the amount of alcohol dehydrogenase with increasing distance from the stomach. In the stomach, the reaction for alcohol dehydrogenase was strongest in the mucus-producing cells, moderate in the parietal cells, and weak in the chief cells. Throughout the gut, alcohol dehydrogenase was present in cells exposed to the lumen in amounts comparable to that of the parietal cells. Crypt cells and goblet cells were negative. The results strongly indicate that alcohol dehydrogenase is an intrinsic component of gastrointestinal epithelial cells.
为阐明乙醇脱氢酶在酒精滥用所致损伤产生过程中可能发挥的作用，了解该酶的确切定位很重要。使用抗乙醇脱氢酶抗体在人体胃肠道组织中对该酶进行定位。在胃肠道各部位的黏膜中均可检测到乙醇脱氢酶。随着与胃距离的增加，乙醇脱氢酶的量显著减少。在胃中，乙醇脱氢酶的反应在黏液分泌细胞中最强，在壁细胞中中等，在主细胞中较弱。在整个肠道中，乙醇脱氢酶存在于暴露于管腔的细胞中，其含量与壁细胞相当。隐窝细胞和杯状细胞呈阴性。结果有力地表明乙醇脱氢酶是胃肠道上皮细胞的固有成分。
