Seitz H K, Egerer G, Simanowski U A, Waldherr R, Eckey R, Agarwal D P, Goedde H W, von Wartburg J P
Department of Medicine, Salem Medical Center, Heidelberg, Germany.
Gut. 1993 Oct;34(10):1433-7. doi: 10.1136/gut.34.10.1433.
As various isoenzymes of gastric alcohol dehydrogenase exist and as the effect of sex and age on these enzymes is unknown, this study measured the activity of gastric alcohol dehydrogenase at high and low ethanol concentrations in endoscopic biopsy specimens from a total of 290 patients of various ages and from 10 patients with chronic alcoholism. Gastric alcohol dehydrogenase was also detected by immunohistological tests in biopsy specimens from 40 patients by the use of a polyclonal rabbit antibody against class I alcohol dehydrogenase. A significant correlation was found between the immunohistological reaction assessed by the intensity of the colour reaction in the biopsy specimen and the activity of alcohol dehydrogenase measured at 580 mM ethanol. While alcohol dehydrogenase activity measured at 16 mM ethanol was not significantly affected by age and sex, both factors influenced alcohol dehydrogenase activity measured at 580 mM ethanol. Young women below 50 years of age had significantly lower alcohol dehydrogenase activities in the gastric corpus and antrum when compared with age matched controls (SEM) (6.4 (0.7) v 8.8 (0.6) nmol/min/mg protein; p < 0.001 and 6.0 (1.3) v 9.5 (1.3) nmol/min/mg protein; p < 0.001). Over 50 years of age this sex difference was no longer detectable, as high Km gastric alcohol dehydrogenase activity decreases with age only in men and not in women. In addition, extremely low alcohol dehydrogenase activities have been found in gastric biopsy specimens from young male alcoholics (2.2 (0.5) nmol/min/mg protein), which returned to normal after two to three weeks of abstinence. The activity of alcohol dehydrogenase in the human stomach measured at 580 mM ethanol is decreased in young women, in elderly men, and in the subject with alcoholism. This decrease in alcohol dehydrogenase activity may contribute to the reduced first pass metabolism of ethanol associated with raised ethanol blood concentrations seen in these people.
由于存在多种胃乙醇脱氢酶同工酶，且性别和年龄对这些酶的影响尚不清楚，本研究测定了来自290名不同年龄患者的内镜活检标本以及10名慢性酒精中毒患者的内镜活检标本在高、低乙醇浓度下胃乙醇脱氢酶的活性。还通过免疫组织学检测，使用抗I类乙醇脱氢酶的多克隆兔抗体，在40名患者的活检标本中检测胃乙醇脱氢酶。在活检标本中通过显色反应强度评估的免疫组织学反应与在580 mM乙醇浓度下测得的乙醇脱氢酶活性之间存在显著相关性。虽然在16 mM乙醇浓度下测得的乙醇脱氢酶活性不受年龄和性别的显著影响，但这两个因素均影响在580 mM乙醇浓度下测得的乙醇脱氢酶活性。与年龄匹配的对照组相比，50岁以下的年轻女性胃体和胃窦中的乙醇脱氢酶活性显著较低（标准误）（6.4（0.7）对8.8（0.6）nmol/分钟/毫克蛋白；p<0.001以及6.0（1.3）对9.5（1.3）nmol/分钟/毫克蛋白；p<0.001）。50岁以上，这种性别差异不再明显，因为高Km胃乙醇脱氢酶活性仅在男性中随年龄降低，而在女性中并非如此。此外，在年轻男性酗酒者的胃活检标本中发现乙醇脱氢酶活性极低（2.2（0.5）nmol/分钟/毫克蛋白），戒酒两到三周后恢复正常。在580 mM乙醇浓度下测得的人胃中乙醇脱氢酶活性在年轻女性、老年男性和酗酒者中降低。乙醇脱氢酶活性的这种降低可能导致这些人群中与乙醇血浓度升高相关的乙醇首过代谢减少。
