Ohishi I, Odagiri Y
Infect Immun. 1984 Jan;43(1):54-8. doi: 10.1128/iai.43.1.54-58.1984.
Botulinum C2 toxin has histopathological activity in the mouse intestine and induces fluid accumulation in intestinal loops. The toxin caused degenerative and necrotic changes in the intestinal mucosa: intracellular vacuolization of epithelial cells, desquamation and necrosis of the villous epithelium, intercellular edema, and infiltration of lymphocytes and histiocytes. The detectable changes in the morphology of the intestinal mucosa preceded the increase in fluid accumulation in intestinal loops. Intraluminal injection of botulinum C2 toxin also induced the leakage of plasma protein into the intestinal lumen as determined by the extravasation of Evans blue. In contrast to botulinum C2 toxin, cholera and Clostridium perfringens enterotoxin controls caused a very slight protein leakage, although these toxins induced marked fluid accumulation in intestinal loops. The results indicate that the mode of action of botulinum C2 toxin in eliciting the secretory response is distinguishable from those of cholera and C. perfringens enterotoxins and suggest that botulinum C2 toxin induces the secretory response by cytopathic effect(s) on the epithelial cells of the intestine.
肉毒杆菌C2毒素在小鼠肠道具有组织病理学活性，并可诱导肠袢积液。该毒素导致肠黏膜发生退行性和坏死性改变：上皮细胞内出现空泡化、绒毛上皮脱落和坏死、细胞间水肿以及淋巴细胞和组织细胞浸润。肠黏膜形态学上可检测到的变化先于肠袢积液增加。通过伊文思蓝外渗测定，肠腔内注射肉毒杆菌C2毒素还可诱导血浆蛋白渗漏至肠腔内。与肉毒杆菌C2毒素相反，霍乱毒素和产气荚膜梭菌肠毒素对照引起的蛋白渗漏非常轻微，尽管这些毒素可诱导肠袢显著积液。结果表明，肉毒杆菌C2毒素引发分泌反应的作用方式与霍乱毒素和产气荚膜梭菌肠毒素不同，提示肉毒杆菌C2毒素通过对肠道上皮细胞的细胞病变效应诱导分泌反应。
