Chen P P, Fong S, Normansell D, Houghten R A, Karras J G, Vaughan J H, Carson D A
J Exp Med. 1984 May 1;159(5):1502-11. doi: 10.1084/jem.159.5.1502.
Antibody against a cross-reactive idiotype (CRI) on human IgM-rheumatoid factor (RF) antibodies was induced by immunization of rabbits with a synthetic peptide ( PSL2 ) corresponding to the second complementarity-determining region (CDR), and adjacent amino acid residues of the kappa light chain of the IgM-RF Sie . The anti-peptide antibody bound efficiently to IgM-RF proteins known to share a cross-reactive idiotype, and to their isolated kappa chains. The anti-CRI was absorbed by, and eluted from, a peptide-Sepharose affinity column. The antibody activity was inhibited by the free peptide in solution. The anti-peptide antibody thus identifies a public idiotype on human IgM-RF, that is largely dependent on the primary sequence of the second CDR of the light chain. Such peptide-induced antiidiotypes of predefined specificity may facilitate studies of the molecular basis of idiotypic cross-reactions, the inheritance and somatic diversification of antibody molecules, and the regulation of the idiotype network.
通过用与IgM - RF Sie κ轻链的第二个互补决定区（CDR）及相邻氨基酸残基相对应的合成肽（PSL2）免疫兔子，诱导出了针对人IgM类风湿因子（RF）抗体上交叉反应独特型（CRI）的抗体。抗肽抗体能有效地与已知具有交叉反应独特型的IgM - RF蛋白及其分离的κ链结合。抗CRI被肽 - 琼脂糖亲和柱吸附并从柱上洗脱。溶液中的游离肽可抑制抗体活性。因此，抗肽抗体识别出了人IgM - RF上的一种公共独特型，该独特型很大程度上依赖于轻链第二个CDR的一级序列。这种具有预定义特异性的肽诱导抗独特型可能有助于研究独特型交叉反应的分子基础、抗体分子的遗传和体细胞多样化以及独特型网络的调节。
