Meseguer M, de Rafael L, Baquero M, Martínez Ferrer M, López-Brea M
Eur J Clin Microbiol. 1984 Apr;3(2):113-5. doi: 10.1007/BF02014327.
A total of 1,592 blood cultures without macroscopic signs of bacterial growth in the first 12-24 h of incubation were processed for both acridine orange stain and blind subculture. One hundred and twenty-one (7.6%) blood cultures were positive by either method; of these, 105 (8.68%) were positive by both methods, 11 (9.1%) positive by acridine orange and negative by subculture, and 5 (4.1%) negative by acridine orange and positive by subculture. The difference between the 116 blood cultures positive by acridine orange and the 110 blood cultures positive by subculture was not statistically significant (p greater than 0.1). Gram stain performed on all acridine orange positive cultures failed to reveal bacteria in 14 cases. Acridine orange staining is a sensitive, rapid and reliable method for detecting bacteria in blood cultures early during incubation. The method is inexpensive and easy to perform and can be substituted for blind subcultures.
对总共1592份在培养的最初12 - 24小时内无细菌生长宏观迹象的血培养物进行吖啶橙染色和盲目传代培养处理。两种方法中共有121份（7.6%）血培养物呈阳性；其中，105份（8.68%）两种方法均呈阳性，11份（9.1%）吖啶橙染色呈阳性而传代培养呈阴性，5份（4.1%）吖啶橙染色呈阴性而传代培养呈阳性。116份吖啶橙染色阳性的血培养物与110份传代培养阳性的血培养物之间的差异无统计学意义（p大于0.1）。对所有吖啶橙染色阳性培养物进行革兰氏染色，有14例未发现细菌。吖啶橙染色是一种在培养早期检测血培养物中细菌的灵敏、快速且可靠的方法。该方法成本低廉且易于操作，可替代盲目传代培养。
