通过吖啶橙染色技术早期检测血培养阳性情况。
Early detection of positive blood cultures by the acridine orange staining technique.
作者信息
Tierney B M, Henry N K, Washington J A
出版信息
J Clin Microbiol. 1983 Oct;18(4):830-3. doi: 10.1128/jcm.18.4.830-833.1983.
Staining 2,205 macroscopically negative blood cultures with acridine orange after 6 to 17 h of inoculation and incubation was as sensitive as an early subculture in detecting positive blood cultures. Of the 179 positive blood cultures, 30 (16.8%) were detected by acridine orange alone, 19 (10.6%) were detected by early subculture alone, 84 (46.9%) were detected by both techniques, and 46 (25.7%) were not detected by either method. The latter group includes cultures that became positive after 48 h of incubation. Acridine orange staining of smears prepared from macroscopically negative blood cultures after 6 to 17 h is a rapid, reliable method to detect positive blood cultures.
对接种并孵育6至17小时后的2205份肉眼观察为阴性的血培养物进行吖啶橙染色，在检测阳性血培养物方面与早期传代培养一样敏感。在179份阳性血培养物中，仅通过吖啶橙检测出30份（16.8%），仅通过早期传代培养检测出19份（10.6%），两种技术均检测出84份（46.9%），两种方法均未检测出46份（25.7%）。后一组包括孵育48小时后才呈阳性的培养物。对接种并孵育6至17小时后肉眼观察为阴性的血培养物制备涂片进行吖啶橙染色，是检测阳性血培养物的一种快速、可靠的方法。
Zotero 插件