源自人IgG1 CH3结构域的一种生物活性肽的分离与鉴定
Isolation and identification of a biologically active peptide derived from the CH3 domain of human IgG1.
作者信息
Morgan E L, Hugli T E, Weigle W O
出版信息
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5388-91. doi: 10.1073/pnas.79.17.5388.
Abstract
A 24-amino acid residue peptide has been isolated from the Fc region of a human IgG1 myeloma protein. The peptide has associated with it the same ability to induce murine B cells to polyclonally secrete antibody as does the intact Fc fragment. Amino acid composition of the peptide indicates that on a mole/mole basis the isolated peptide is identical to that published for residues 335-358 in the Eu IgG1 sequence. This peptide corresponds roughly to the first 24 amino acids of the CH3 domain. It is believed that the immunoregulatory properties that have been ascribed to the Fc fragment are associated with this peptide.
摘要
一种由24个氨基酸残基组成的肽已从人IgG1骨髓瘤蛋白的Fc区域分离出来。该肽与完整的Fc片段一样，具有诱导鼠B细胞多克隆分泌抗体的能力。该肽的氨基酸组成表明，在摩尔/摩尔基础上，分离出的肽与已发表的Eu IgG1序列中335 - 358位残基相同。此肽大致对应于CH3结构域的前24个氨基酸。据信，归因于Fc片段的免疫调节特性与该肽有关。
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本文引用的文献
1
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Science. 1963 Apr 26;140(3565):405. doi: 10.1126/science.140.3565.405.
2
The hydrolysis of rabbit y-globulin and antibodies with crystalline papain.用结晶木瓜蛋白酶对兔γ球蛋白和抗体进行水解。
Biochem J. 1959 Sep;73(1):119-26. doi: 10.1042/bj0730119.
3
4
Regulation of the immune response. I. The potentiation of in vivo and in vitro immune responses by Fc fragments.免疫反应的调节。I. Fc片段对体内和体外免疫反应的增强作用。
J Exp Med. 1980 Jul 1;152(1):113-23. doi: 10.1084/jem.152.1.113.
5
The interaction of the Facb fragment of rabbit anti-sheep red cell IgG with guinea pig macrophages, and human monocytes and granulocytes.
Mol Immunol. 1982 Feb;19(2):247-52. doi: 10.1016/0161-5890(82)90337-6.
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