Landay A, Poon M C, Clement L T, Grossi C E
J Clin Immunol. 1984 Jul;4(4):326-34. doi: 10.1007/BF00915301.
In this study we have identified and characterized an expanded granular lymphocyte population in a patient with anemia and granulocytopenia. Granular lymphocytes were identified through the presence of cytoplasmic azurophilic granules, the dispersed granular pattern of cytochemical staining for acid hydrolases, and the ultrastructural localization of acid phosphatase within the granules. The surface phenotype of the granular lymphocytes was E+, FcR-, Leu 4+, Leu 2+, D 12+ (Leu 15+), OKM1+, and Leu 7+. This phenotype has not been reported previously in patients with similar features. Functional studies on FACS-purified populations showed that the patient's granular lymphocytes responded poorly to T-cell mitogens and were inefficient in NK and ADCC assays but exerted a potent suppressor effect on both T-cell proliferation and B-cell differentiation. The phenotype and functions of the expanded granular lymphocyte population correspond to those of a subset of normal E rosette-forming granular lymphocytes.
在本研究中，我们在一名患有贫血和粒细胞减少症的患者体内鉴定并描述了一种扩增的颗粒淋巴细胞群体。通过细胞质嗜天青颗粒的存在、酸性水解酶细胞化学染色的分散颗粒模式以及酸性磷酸酶在颗粒内的超微结构定位来鉴定颗粒淋巴细胞。颗粒淋巴细胞的表面表型为E +、FcR -、Leu 4 +、Leu 2 +、D 12 +（Leu 15 +）、OKM1 +和Leu 7 +。这种表型在具有类似特征的患者中以前尚未见报道。对经荧光激活细胞分选仪（FACS）纯化的细胞群体进行的功能研究表明，该患者的颗粒淋巴细胞对T细胞有丝分裂原反应较差，在自然杀伤（NK）和抗体依赖的细胞介导的细胞毒性（ADCC）试验中效率低下，但对T细胞增殖和B细胞分化均具有强大的抑制作用。扩增的颗粒淋巴细胞群体的表型和功能与正常E花环形成颗粒淋巴细胞亚群的表型和功能一致。
