焦磷酸钠对逆转录酶介导的RNA.DNA杂交体降解的抑制作用
Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase.
作者信息
Myers J C, Spiegelman S
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5329-33. doi: 10.1073/pnas.75.11.5329.
Abstract
Sodium pyrophosphate inhibits synthesis of anticomplementary DNA during a reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) catalyzed reaction. In the presence of pyrophosphate, the complementary DNA remains stably complexed to the RNA template. In the absence of pyrophosphate, the DNA. RNA hybrid template is degraded and anticomplementary DNA is synthesized. High concentrations of additives containing phosphodiester bonds appear to inhibit the ribonuclease H activity (hybrid nuclease, EC 3.1.4.34) of the reverse transcriptase, therby preventing formation of RNA primers necessary for the synthesis of anticomplementary DNA.
摘要
焦磷酸钠在逆转录酶（RNA 指导的 DNA 核苷酸转移酶，EC 2.7.7.7）催化的反应过程中抑制反互补 DNA 的合成。在焦磷酸存在的情况下，互补 DNA 与 RNA 模板保持稳定结合。在没有焦磷酸的情况下，DNA-RNA 杂交模板会降解，反互补 DNA 得以合成。高浓度含磷酸二酯键的添加剂似乎会抑制逆转录酶的核糖核酸酶 H 活性（杂交核酸酶，EC 3.1.4.34），从而阻止合成反互补 DNA 所需的 RNA 引物的形成。
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