通过酶联免疫吸附试验阻断法检测轮状病毒抗体。
Measurement of rotavirus antibody by an enzyme-linked immunosorbent assay blocking assay.
作者信息
Yolken R H, Wyatt R G, Barbour B A, Kim H W, Kapikian A Z, Chanock R M
出版信息
J Clin Microbiol. 1978 Sep;8(3):283-7. doi: 10.1128/jcm.8.3.283-287.1978.
Abstract
A new method for the measurement of rotavirus antibody is described, utilizing the system of enzyme-linked immunosorbent assay (ELISA). In this method, serum is incubated with a fixed amount of rotavirus antigen, and the amount of antibody is determined by measuring the amount of unneutralized antigen. Such an assay system proved to be as efficient as the other available rotaviral antibody systems. The ELISA blocking assay also has the advantages of not requiring purified or gnotobiotic antigen and of being able to measure rotaviral antibody in all animal species.
摘要
本文描述了一种利用酶联免疫吸附测定（ELISA）系统测量轮状病毒抗体的新方法。在该方法中，血清与固定量的轮状病毒抗原一起孵育，通过测量未中和抗原的量来确定抗体的量。事实证明，这种检测系统与其他现有的轮状病毒抗体系统一样有效。ELISA阻断检测还具有不需要纯化抗原或无菌抗原以及能够测量所有动物物种中轮状病毒抗体的优点。
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