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一种来自具核梭杆菌4H的限制性内切酶,其识别GCNGC。

A restriction enzyme from Fusobacterium nucleatum 4H which recognizes GCNGC.

作者信息

Leung D W, Lui A C, Merilees H, McBride B C, Smith M

出版信息

Nucleic Acids Res. 1979 Jan;6(1):17-25. doi: 10.1093/nar/6.1.17.

DOI:10.1093/nar/6.1.17
PMID:424288
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327670/
Abstract

A site-specific restriction endonuclease Fnu4H I isolated from Fusobacterium nucleatum 4H recognizes the DNA nucleotide sequence 5'G C N G C-3'/3'-C G N C G-5' and cleaves as indicated by the arrows.

摘要

从具核梭杆菌4H中分离出的位点特异性限制性内切酶Fnu4H I识别DNA核苷酸序列5'G C N G C-3'/3'-C G N C G-5',并按箭头所示进行切割。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/327670/d1eb58f8e36d/nar00442-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/327670/db536804d078/nar00442-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/327670/8b8066bf4712/nar00442-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/327670/60ded88f6c53/nar00442-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/327670/d1eb58f8e36d/nar00442-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/327670/db536804d078/nar00442-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/327670/8b8066bf4712/nar00442-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/327670/60ded88f6c53/nar00442-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9965/327670/d1eb58f8e36d/nar00442-0033-a.jpg

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引用本文的文献

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PLoS One. 2017 Jun 27;12(6):e0179853. doi: 10.1371/journal.pone.0179853. eCollection 2017.
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Efficient gene transfer and targeted mutagenesis in Fusobacterium nucleatum.具核梭杆菌中的高效基因转移和靶向诱变
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Native plasmids of Fusobacterium nucleatum: characterization and use in development of genetic systems.

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A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.一种通过DNA聚合酶引发合成来测定DNA序列的快速方法。
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Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
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A new specific endonuclease present in Xanthomonas holcicola, Xanthomonas papavericola and Brevibacterium luteum.一种存在于玉米黄单胞菌、罂粟黄单胞菌和黄色短杆菌中的新型特异性核酸内切酶。
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