用于定量检测单纯疱疹病毒和巨细胞病毒抗体的固相免疫荧光法评估
Evaluation of solid-phase immunofluorescence for quantitation of antibodies to herpes simplex virus and cytomegalovirus.
作者信息
Benjamin W R, Specter S C, Klein T W, Hitchings M, Friedman H
出版信息
J Clin Microbiol. 1980 Oct;12(4):558-61. doi: 10.1128/jcm.12.4.558-561.1980.
A recently developed semiautomated technique based on solid-phase immunofluorescence (FIAX) was compared with complement fixation for the determination of antibody levels to herpes simplex virus and cytomegalovorus in human serum samples. The results demonstrated that the FIAX method was in aggreement with the complement fixation technique for 97% of the serum samples tested. Reproducible titers were obtained from multiple FIAX determinations of representative sera within the same experimental run and between separate experimental runs. However, variability was rather high for patient sera with low (less than or equal to 1:5) antibody levels to cytomegalovirus. Hence, the results obtained by the FIAX technique were reproducible, and the FIAX system was as sensitive as complement fixation for the determination of antibody levels to herpes simplex virus and cytomegalovirus.
将一种最近开发的基于固相免疫荧光的半自动技术（FIAX）与补体结合试验进行比较，以测定人血清样本中针对单纯疱疹病毒和巨细胞病毒的抗体水平。结果表明，对于97%的测试血清样本，FIAX方法与补体结合技术结果一致。在同一实验批次内以及不同实验批次之间，对代表性血清进行多次FIAX测定可获得可重复的滴度。然而，对于巨细胞病毒抗体水平较低（小于或等于1:5）的患者血清，变异性相当高。因此，FIAX技术获得的结果具有可重复性，并且在测定针对单纯疱疹病毒和巨细胞病毒的抗体水平方面，FIAX系统与补体结合试验一样敏感。
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