Kaushik A K, Pandey R
Immunology. 1980 Sep;41(1):153-8.
The experiments on protective immunity were conducted in a closely bred population of mice which did not show graft versus host reactions. Simultaneous passive transfer of 0.25 ml rabbit anti-buffalopox virus serum and subsequent challenge with 0.05 ml 10(5) TCID100/ml of buffalopox virus (BPV) showed 57.15 and 47.06% protection with a 1:2 and 1:16 dilutions of buffalopox hyperimmune serum 24 h prior to challenge with BPV showed 87.50 and 75.0% protection, respectively. The passive transfer of normal saline or normal rabbit serum did not protect mice against lethal challenge with BPV. The protection conferred by 5.0 x 10(6) and 15.0 x 10(6) spleen cells obtained from immune donor mice was 37.5, 42.85 and 50.0%, respectively. None of the mice that received spleen cells obtained from donors immunized with normal saline emulsified in Freund's incomplete adjuvant survived lethal challenge with BPV. T- and B-cell levels in the peripheral blood of rabbits during the course of BPV infection revealed transient relative lymphopaenia on the 4th, 5th and 7th days post infection. These values returned to normal on the 14th and 21st days post infection. No marked difference in percentage of B cells or absolute B-cell number between control and infected rabbits was found. This study revealed that both cellular and humoral immunity seem to play a role in recovery from BPV infection in mice and rabbits.
在一个不发生移植物抗宿主反应的近交系小鼠群体中进行了保护性免疫实验。同时被动转移0.25 ml兔抗水牛痘病毒血清，随后用0.05 ml 10(5) TCID100/ml的水牛痘病毒（BPV）进行攻击，结果显示，在攻击前24小时用1:2和1:16稀释的水牛痘超免疫血清分别有57.15%和47.06%的保护率；而在攻击前24小时用1:2和1:16稀释的水牛痘超免疫血清分别有87.50%和75.0%的保护率。被动转移生理盐水或正常兔血清不能保护小鼠免受BPV的致死性攻击。从免疫供体小鼠获得的5.0 x 10(6)和15.0 x 10(6)个脾细胞提供的保护率分别为37.5%、42.85%和50.0%。接受用弗氏不完全佐剂乳化的生理盐水免疫的供体小鼠的脾细胞的小鼠，无一存活BPV的致死性攻击。在BPV感染过程中，兔外周血中的T细胞和B细胞水平显示，感染后第4、5和7天出现短暂的相对淋巴细胞减少。这些数值在感染后第14天和21天恢复正常。未发现对照兔和感染兔之间B细胞百分比或绝对B细胞数量有明显差异。这项研究表明，细胞免疫和体液免疫似乎在小鼠和兔从BPV感染中恢复的过程中都发挥了作用。
