Tamura T, Chiba S, Chiba Y, Nakao T
Infect Immun. 1980 Sep;29(3):842-5. doi: 10.1128/iai.29.3.842-845.1980.
The local secretory immune mechanism in infants with cytomegalovirus infection was studied by a measurement of neutralizing antibody in saliva. Neutralizing antibodies were determined by the microculture plaque assay in 65 saliva specimens including 54 samples from cytomegalovirus-infected subjects and 11 from seronegative controls. In addition, cytomegalovirus isolation from saliva or urine or both and antibody determination in serum and saliva were simultaneously performed on seven infants with cytomegalovirus excretion over long periods. Results obtained were as follows. (i) Neutralizing antibodies were detected in 41 (76%) of 54 saliva specimens obtained from infected subjects but in none of the 11 seronegative controls. (ii) Neutralizing antibodies in saliva were of low titer but persistently detectable in all but one of the seven infants. No relationship was recognized between the cessation of virus excretion and the development of neutralizing antibodies in saliva. (iii) Virus-neutralizing activity was specifically found in the immunoglobulin A fraction of pooled saliva by diethylaminoethyl cellulose chromatography.
通过检测唾液中的中和抗体，研究了巨细胞病毒感染婴儿的局部分泌免疫机制。采用微量培养蚀斑试验，对65份唾液标本进行了中和抗体检测，其中包括54份来自巨细胞病毒感染受试者的样本和11份来自血清阴性对照的样本。此外，对7例长期排泄巨细胞病毒的婴儿，同时进行了唾液或尿液或两者的巨细胞病毒分离以及血清和唾液中的抗体检测。结果如下：（i）在54份来自感染受试者的唾液标本中，有41份（76%）检测到中和抗体，而11份血清阴性对照中均未检测到。（ii）唾液中的中和抗体效价较低，但在7例婴儿中，除1例之外，其余婴儿的中和抗体均可持续检测到。未发现病毒排泄停止与唾液中中和抗体产生之间存在关联。（iii）通过二乙氨基乙基纤维素层析法，在混合唾液的免疫球蛋白A组分中特异性地发现了病毒中和活性。
