Gregory W W, Menegus M A
J Clin Microbiol. 1983 Oct;18(4):1003-5. doi: 10.1128/jcm.18.4.1003-1005.1983.
The sensitivity of WI-38 cell monolayers for the isolation of cytomegalovirus from fresh clinical specimens was reduced by 41% when cultures were incubated at 33 rather than at 36 degrees C. The mean time to initial detection of cytomegalovirus cytopathic effects in cultures incubated at 33 degrees C was 4.3 days later than in cultures incubated at 36 degrees C (4.0 and 5.4 days later for saliva and urine cultures, respectively). Thus, incubation of cultures at 33 degrees C seriously compromises the efficiency with which cytomegalovirus can be isolated from clinical specimens.
当将WI-38细胞单层培养物在33℃而非36℃下孵育时，从新鲜临床标本中分离巨细胞病毒的敏感性降低了41%。在33℃下孵育的培养物中，首次检测到巨细胞病毒细胞病变效应的平均时间比在36℃下孵育的培养物晚4.3天（唾液和尿液培养物分别晚4.0天和5.4天）。因此，在33℃下孵育培养物会严重损害从临床标本中分离巨细胞病毒的效率。
