Vartdal F, Vandvik B, Norrby E
Ann Neurol. 1980 Sep;8(3):248-55. doi: 10.1002/ana.410080305.
An imprint electroimmunofixation method (IEIF) was used to characterize antibodies to eight viral antigens (measles, mumps, rubella, herpes simplex type 1, varicella-zoster, vaccinia, cytomegalovirus, adenovirus) and four bacterial antigens (beta-hemolytic streptococcus, Hemophilus influenzae type B, Escherichia coli, enterococcus) in serum and cerebrospinal fluid (CSF) of 12 patients with multiple sclerosis (MS). Twelve patients matched for age and sex sex served as controls. Evidence for intrathecal synthesis of oligoclonal antibodies to one or more antigens was found in all 12 MS patients and in 1 of the controls. In the MS group, antibodies to viruses with neurotropic properties were more frequently associated with local synthesis than antibodies to other viruses and bacteria. The types and number of locally synthesized antibodies showed no correlation with disease duration and severity. The antibodies were not associated with oligoclonal CSF IgG and appear to account for only a minor fraction of the locally synthesized CSF IgG in MS.
采用印记免疫固定电泳法（IEIF）对12例多发性硬化症（MS）患者血清和脑脊液（CSF）中针对8种病毒抗原（麻疹、腮腺炎、风疹、1型单纯疱疹病毒、水痘-带状疱疹病毒、牛痘病毒、巨细胞病毒、腺病毒）和4种细菌抗原（β溶血性链球菌、B型流感嗜血杆菌、大肠杆菌、肠球菌）的抗体进行特性分析。选取12例年龄和性别匹配的患者作为对照。在所有12例MS患者及1例对照中均发现有鞘内合成针对一种或多种抗原的寡克隆抗体的证据。在MS组中，与具有嗜神经特性的病毒相关的抗体比针对其他病毒和细菌的抗体更常与局部合成相关。局部合成抗体的类型和数量与病程及严重程度无关。这些抗体与脑脊液寡克隆IgG无关，似乎仅占MS患者局部合成脑脊液IgG的一小部分。
