Polak Justyna, Wagnerberger Johanna H, Torsetnes Silje Bøen, Lindeman Ida, Høglund Rune A Aa, Vartdal Frode, Sollid Ludvig M, Lossius Andreas
Department of Immunology, Oslo University Hospital, University of Oslo, Oslo, Norway.
K.G. Jebsen Coeliac Disease Research Centre, University of Oslo, Oslo, Norway.
Front Cell Neurosci. 2023 Jun 8;17:1189709. doi: 10.3389/fncel.2023.1189709. eCollection 2023.
The phenotypes of B lineage cells that produce oligoclonal IgG in multiple sclerosis have not been unequivocally determined. Here, we utilized single-cell RNA-seq data of intrathecal B lineage cells in combination with mass spectrometry of intrathecally synthesized IgG to identify its cellular source. We found that the intrathecally produced IgG matched a larger fraction of clonally expanded antibody-secreting cells compared to singletons. The IgG was traced back to two clonally related clusters of antibody-secreting cells, one comprising highly proliferating cells, and the other consisting of more differentiated cells expressing genes associated with immunoglobulin synthesis. These findings suggest some degree of heterogeneity among cells that produce oligoclonal IgG in multiple sclerosis.
在多发性硬化症中产生寡克隆IgG的B淋巴细胞系细胞的表型尚未得到明确确定。在此，我们利用鞘内B淋巴细胞系细胞的单细胞RNA测序数据，并结合鞘内合成IgG的质谱分析来确定其细胞来源。我们发现，与单细胞相比，鞘内产生的IgG与克隆扩增的抗体分泌细胞的比例更高。IgG可追溯到两个克隆相关的抗体分泌细胞簇，一个包含高度增殖的细胞，另一个由表达与免疫球蛋白合成相关基因的更分化细胞组成。这些发现表明，在多发性硬化症中产生寡克隆IgG的细胞之间存在一定程度的异质性。
