小鼠白血病病毒II的RNA代谢。未感染的BALB/c细胞系JLS-V9中的内源性病毒特异性RNA。
RNA metabolism of murine leukemia virus II. Endogenous virus-specific RNA in the uninfected BALB/c cell line JLS-V9.
作者信息
Fan H, Besmer P
出版信息
J Virol. 1975 Apr;15(4):836-42. doi: 10.1128/JVI.15.4.836-842.1975.
Abstract
Type C virus-specific RNA sequences of BALB/c endogenous virus were detected in JLS-V9 cells (an uninfected BALB/c derived line) by annealing cell RNA with 3-H-labeled virus-specific DNA. Endogenous viruses used in preparing the 3-H-labeled DNA (mostly xenotropic) was prepared from JLS-V9 cells induced to produce virus with iododeoxyuridine. In whole-cell extracts, two virus-specific RNA species, 38S and 27S, were detected. No 60 to 70S virus-specific RNA was found. The same two species of virus-specific RNA were observed in isolated cytoplasmic RNA and in cytoplasmic RNA selected for polyadenylic acid-containing species by binding and elution from oligo(dT) cellulose. Very little, if any, of the virus-specific RNA was active as messenger RNA on polyribosomes. No virus-specific RNA transcribed from genes coding for the BALB/c endogenous N-tropic virus was detected, since 3-H-labeled DNA prepared from endogenous N-tropic virus did not hybridize measurably with JLS-V9 RNA.
摘要
通过使细胞RNA与³H标记的病毒特异性DNA退火，在JLS - V9细胞（一个未感染的源自BALB/c的细胞系）中检测到了BALB/c内源性病毒的C型病毒特异性RNA序列。用于制备³H标记DNA（大多为嗜异性）的内源性病毒是从用碘脱氧尿苷诱导产生病毒的JLS - V9细胞中制备的。在全细胞提取物中，检测到了两种病毒特异性RNA种类，即38S和27S。未发现60至70S的病毒特异性RNA。在分离的细胞质RNA以及通过与寡聚（dT）纤维素结合和洗脱而选择的含聚腺苷酸种类的细胞质RNA中，观察到了相同的两种病毒特异性RNA。病毒特异性RNA作为信使RNA在多核糖体上的活性非常低（如果有的话）。未检测到从编码BALB/c内源性N - 嗜性病毒的基因转录的病毒特异性RNA，因为从内源性N - 嗜性病毒制备的³H标记DNA与JLS - V9 RNA没有可测量的杂交。
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