Rosenberg Z F, Pedersen F S, Haseltine W A
J Virol. 1980 Aug;35(2):542-6. doi: 10.1128/JVI.35.2.542-546.1980.
The genomes of several strains of feline leukemia virus (FeLV) were compared by two-dimensional polyacrylamide gel electrophoresis of the large RNase T1-resistant oligonucleotides of the 70S RNA. Differences between each strain of FeLV tested were detected by this method. We estimate that the degree of sequence identity between the viruses is: FeLV A (Glasgow-1) to FeLV B (Snyder-Theilen), 52%; FeLV A (Glasgow-1) to FeLV C(Sarma), 66%; FeLV B(Snyder-Theilen) to FeLV C (Sarma), 37%. The fingerprints of two independent isolates of FeLV strains of subgroup A (Glasgow-1 and Rickard) were detectably different. We conclude that the RNase T1 oligonucleotide fingerprint pattern provides a useful tool for identification of FeLV strains.
通过对70S RNA的大核糖核酸酶T1抗性寡核苷酸进行二维聚丙烯酰胺凝胶电泳，比较了几种猫白血病病毒（FeLV）毒株的基因组。用这种方法检测了所测试的每种FeLV毒株之间的差异。我们估计这些病毒之间的序列同一性程度为：FeLV A（格拉斯哥-1）与FeLV B（斯奈德-泰伦），52%；FeLV A（格拉斯哥-1）与FeLV C（萨尔马），66%；FeLV B（斯奈德-泰伦）与FeLV C（萨尔马），37%。A亚组FeLV毒株（格拉斯哥-1和里卡德）的两个独立分离株的指纹图谱有明显差异。我们得出结论，核糖核酸酶T1寡核苷酸指纹图谱模式为鉴定FeLV毒株提供了一种有用的工具。
