Hornbeck C L, Gravelle C R, Hollinger F B, Bradley D W
Intervirology. 1975;6(4-5):309-14. doi: 10.1159/000149484.
Virus-like particles shown to be associated with hepatitis A infection were recently visualized by immune electron microscopy in human and chimpanzee acute-phase fecal extracts. Agarose gel filtration of concentrated chimpanzee fecal extracts containing those 27-nm diameter particles separated more than 99% of the high molecular weight fecal impurities into two major peaks as determined by absorbance at 280 and 260 nm. The hepatitis A-associated particles were found between these two peaks by both immune electron microscopy and a new immunoradiometric assay.
最近，通过免疫电子显微镜在人和黑猩猩的急性期粪便提取物中观察到了与甲型肝炎感染相关的病毒样颗粒。对含有这些直径为27纳米颗粒的浓缩黑猩猩粪便提取物进行琼脂糖凝胶过滤，根据在280和260纳米处的吸光度测定，将99%以上的高分子量粪便杂质分离成两个主要峰。通过免疫电子显微镜和一种新的免疫放射分析方法，在这两个峰之间发现了与甲型肝炎相关的颗粒。
