Niilo L, Bainborough A R
Can J Microbiol. 1980 Sep;26(9):1162-4. doi: 10.1139/m80-192.
Sera from human, cattle, sheep, swine, and horse populations in western Canada were tested for the presence of Clostridium perfringens enterotoxin antibody by the passive hemagglutination (PHA) test, supplemented by an immunodiffusion test and by counterimmunoelectrophoresis. A total of 224 human, 345 cattle, 165 sheep, 620 swine, and 768 horse serum samples were examined. Low-titer reactions in the PHA test were detected in human, cattle, horse, and swine sera, in that order, with no titers demonstrated in sheep. The titers in human sera ranged up to 1:128 and three of these samples were also positive in the other two serological tests. The significance of this antibody is not clear, but it is suggested that the low prevalence of the antibody may reflect a low prevalence of enterotoxigenic C. perfringens strains in western Canada. Such serological surveys may be applicable to epidemiological studies involving enterotoxigenic C perfringens.
采用被动血凝试验（PHA），并辅以免疫扩散试验和对流免疫电泳，检测了加拿大西部人群、牛群、羊群、猪群和马群血清中是否存在产气荚膜梭菌肠毒素抗体。共检测了224份人血清、345份牛血清、165份羊血清、620份猪血清和768份马血清样本。在PHA试验中，人、牛、马和猪血清依次检测到低滴度反应，而羊血清未显示滴度。人血清中的滴度最高可达1:128，其中三份样本在其他两项血清学试验中也呈阳性。这种抗体的意义尚不清楚，但有人认为，抗体的低流行率可能反映了加拿大西部产肠毒素产气荚膜梭菌菌株的低流行率。此类血清学调查可能适用于涉及产肠毒素产气荚膜梭菌的流行病学研究。
