Shirley J A, Beards G M, Thouless M E, Flewett T H
Arch Virol. 1981;67(1):1-9. doi: 10.1007/BF01314596.
The influence of divalent cations on the stability of human rotavirus was investigated using the indirect immunofluorescence (FA) technique in LLC-MK2 cells to titrate infectivity. Rotavirus infectivity was stabilized by calcium and strontium ions in solution but not by magnesium ions. Rotavirus isolates were found to be much less stable at 37 degrees C than at + 4 degrees C or 20 degrees. A study of virus morphology at intervals during the course of the experiment and treatment with the chelating agents EDTA and EGTA suggests that loss of infectivity coincides with the removal of the outer capsid layer and that calcium may be required to maintain virus integrity.
利用间接免疫荧光（FA）技术在LLC-MK2细胞中滴定感染性，研究了二价阳离子对人轮状病毒稳定性的影响。溶液中的钙和锶离子可稳定轮状病毒的感染性，但镁离子则不能。发现轮状病毒分离株在37℃时比在4℃或20℃时稳定性差得多。在实验过程中定期对病毒形态进行研究，并使用螯合剂乙二胺四乙酸（EDTA）和乙二醇双四乙酸（EGTA）进行处理，结果表明感染性的丧失与病毒外膜层的去除同时发生，并且可能需要钙来维持病毒的完整性。
